1. Immunohistochemical Study of the Immunoreactive Follicle Stimulating Hormone (ir-FSH) Cells Distribution in Pituitary Gland of Rat(Rattus norvegicus)

Patrick Flaggellata, Sri Wahyuni, Muhammad Jalaluddin, Hamny Hamny, Gholib Gholib, T. Armansyah, TR, Muslim Akmal


The objective of this study was to identified the development of the immunoreactive follicle stimulating hormone (ir-FSH) cells in pituitary glands of rats (Rattus norvegicus)in different age using immunohistochemical (IHC) methods. The pituitary organ that used in this study was collected from eight white female rats aged 2, 4, 6, and >12 months. Pituitary glands were processed histologically and stained with IHC methods to detect the ir-FSH cells. The result showed that the ir-FSH cells were observed in pars distalis (PD), pars tuberalis (PT), and pars intermedia (PI) of adenohypophysis with different number of cells. Furthermore, ir-FSH cells were found abundantly (+++) in the PT and a few (+) in PD of rats aged two months. In rats aged 4 months those cells were distributed in slight number (+) in PT and abundant (+++) in PD. In addition, the cells were also found in a great quantities in rats whose ages were 6 months, (+++) in both PT and PD, but these cells decreased (++) in rats aged >12 months in PD and absent (-) in PT. In conclusion, the devalopment and distribution of ir-FSH cells were found in pars tuberalis and pars distalis adenohypophysis in the pituitary glands of rats aged 2 up to >12 months with different number and distribution patterns.


ir-FSH cells; immunohistochemistry; adenohypophysis; and Rattus norvegicus

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Brown, R.E. 1994. An Introduction to Neuroendocrinology. Cambridge University Press, United Kingdom.

Busman, H. dan M. Biomed. 2013. Histologi ulas vagina dan waktu siklus estrus masa subur mencit betina setelah pemberian ekstrak rimpang rumput teki. Prosiding Semirata FMIPA Universitas Lampung. 371-375.

Dyce, K.M., W.O. Sack, and C.J.G. Wensing. 1996. Text Book of Veterinary Anatomy, 2nd ed. WB. Sunders, Philadelphia.

Hafez, E.S.E.2000. Female Reproductive Organs. In Reproduction and Breeding Techniques for Laboratory Animal. 3rd ed. Lea and Febiger. Philadelphia.

Hullinger, R, l, and O. M. Andrisani. 2006. Endocrine System. In Dellmann’s textbook of Veterinary Histology. 6th ed. Jo Ann Eurell and Brian L. Frappier. Blackwell Publishing, USA.

Inoue, K, and K. Kurosumi. 1984. Ultrastructural immunocytochemical localizationof LH and FSH in the pituitary of the untreated male rat. Cell Tissue. Res. 235:77–83.

Key, M. 2009. Immunohistochemistry Staining Methode. In Education Guide: Immunohistochemical Stain Methode. 5th ed. George Kumar and Lars Rudbeck. Dako, California.

Kieran, J.A. 1990. Histologycal and Histochemical Methode: Theory and Praktice. 2nd ed. Pergamon Press, New York.

Larasaty, W. 2013. Uji Antifertilitas Ekstrak Etil Asetat Biji Jarak Pagar (Jatropha Curcas L.) pada Tikus Putih Jantan (Rattus norvegicus) Galur Sparague Dawley Secara In Vivo. Skripsi. Program Studi Farmasi, UIN Syarif Hidayatullah, Jakarta.

Pandolfi, M., F.L.L. Nostro, A. Shimizu, A.G. Pozzi, F.J. Meijide. G.R. Vazques, and M.C. Maggese. 2006. Identification of immunoreactive FSH and LH cells in the Cichild Fish Cichlasoma Dimerus during the ontogeny and sexual differentiation. Anat. Embryol. DOI 10.1007/ s00429-006-86-9.

Partodiharjo, S. 1980. Ilmu Reproduksi Hewan. Penerbit Mutiara, Jakarta.

Proudman, J.A., F. Vandesande, and L.R. Berghman. 1999. Immunohistochemical evidence that follicle stimulating hormone and luteinizing hormone residen in separate cells in the chicken pituitary. Biology of Reproduction. 60: 1324-1328.

Reimers, T, J. 2003. The Pituitary Gland. in McDonald’s Veterinary Endocrinology and Reproduction.

th ed. Mauricio H. Pineda and Micheal P. Dooley. Lowa State Press, USA.

Rose, M.P., E.G.D. Rose, and H.B. Adam. 2000. Definition and measurement of follicle stimulating hormone. Endocrine Reviews. 21(1):5–22.

Sasaki, F. and S. Nishioka. 1998. Fetal development of the pituitary gland in the beagle. The Anatomical Record. 251:143-151.

Sasaki, F., Y. Ichikawa, and S. Yamauchi. 1992. Immunohistological analysis in the distribution of

cells in the fetal porcine adenohypophysis. The Anatomical Record. 233:135-142.

Taylor, C.R. 2006. Techniques Of Immunohistochemistry: Principles, Pitfalls and Standardization. In Dabbs D (Ed). Diagnostic Immunohistochemistry. 2nd ed. Elsevier Churchill Livingstone, Philadelphia.

Vector Lab. 2014. Instruction For Immunohistochemical Staining Using Goat Primary Antibodies. http://www.vectorlabs.com. (27 November 2015).

DOI: https://doi.org/10.21157/j.med.vet..v12i1.4092

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