2. Antibacterial Test of Red Dragon Fruit Extract Peel (Hylocereus polyrhizus) Against Bacteria Salmonella pullorum
Abstract
The purpose of this study is to determine the effect of the red dragon fruit peels (Hylocereus polyrhizus) extract on the growth of Salmonella pullorum. This research was conducted at the Laboratory of Microbiology, Veterinary Faculty, University of Syiah Kuala, Banda Aceh. Red dragon fruit peels that used in this study is obtained from ripe fruit and Salmonella pullorum cultures is obtained from Microbiology Laboratory of Veterinary Faculty Unsyiah. Salmonella pullorum culture added evenly on the surface of Mueller Hinton Agar, the number of the bacteria have been standarized using Mc Farland'1 method. Discs soaked in the red dragon fruit peels extracts at different concentrations (20 mg/ml, 40 mg/mL, and 60 mg/ml). Ampicillin is used as positive controls and DMSO (dimethyl sulfoxide) was placed on the surface of Mueller Hinton Agar. The parameters in this study is the inhibition zone diameter. Data were analyzed descriptively. The results showed that the red dragon fruit peel extract is able to inhibit the growth of Salmonella pullorum and the average of inhibition diameter zone at a concentration 60 mg / ml is 9.6 mm, 40 mg / ml is 9.4 mm and 20 mg / ml is 9.3 mm
Keywords
Full Text:
PDFReferences
Amalia, S., S. Wahdaningsih, and E.K. Untari. 2014. Antibacterial activity testing of n-Hexane fraction of red dragon (Hylocereus polyrhizus Britton dan Rose) fruit peel on Staphylococcus aureus ATCC 25923. Traditional Medicine Journal. 19(2):89-94.
Berchieri Jr, A., C.K. Murphy, K. Marston, and P.A. Barrow. 2001. Observations on the persistence and vertical transmission of Salmonella enterica serovars Pullorum and Gallinarum in chickens: effect of bacterial and host genetic background. Avian Pathology. 30(3):221-231.
Cappuccino, J and N. Sherman. 1987. Microbiology: A Laboratory Manual. Fourth Edition. P. 60, 139, 186, 471. Addison-Wesley Publishing Company : New York.
Charlton, B.R. 2000. Avian Disease Manual. 5th ed. American Association of Avian Pathologists, University of Pennsylvania, USA.
Cowan, M.M. 1999. Plant products as antimicrobial agents. Clinical Microbiology Reviews. 12(4):564-582.
Davis, W.W and T.R. Stout. 1971. Disc plate methods of microbiological antibiotic assay. Journal of Applied Microbiology. 22(4):659-665.
Dewoto, H.R. 2007. Pengembangan obat tradisional Indonesia menjadi fitofarmaka. Majalah Kedokteran Indonesia. 57(7):205-211.
Direktorat Kesehatan Hewan. 2014. Manual Penyakit Hewan Unggas. Edisi 2. Penerbit Direktorat Jenderal Peternakan dan Kesehatan Hewan, Kementerian Pertanian RI, Jakarta.
Dodson, S.V., J.J. Maurer, P.S. Holt and M.D. Lee. 1999. Temporal changes in the populations genetics of Salmonella Pullorum . Avian Diseases. 43(4):685-695.
Hossain, M.S., E.H. Chowdhury, M.M. Islam, M.G. Haider, and M.M. Hossain. 2006. Avian salmonella infection: isolation and identification of organisms and histopathological study. Bangladesh Journal of Veterinary Medicine. 4(1):7-12.
Izzati, M. 2007. Skreening potensi antibakteri pada beberapa spesies rumput laut terhadap bakteri patogen pada udang windu. BIOMA. 9(2):62-67.
Jaafar, R.A., M. Nazri, and W. Khairuddin. 2009. Proximate analysis of dragon fruit (Hylecereus polyrhizus). American Journal of Applied Sciences. 6(7):1341-1346.
Jawetz, E., J.L. Melnick, and E.A. Andelberg. 1982. Mikrobiologi Untuk Profesi Kesehatan. Penerjemah: G. Bonang. Edisi 6. EGC, Penerbit Buku Kedokteran, Jakarta.
Khachatryan, A.R., T.E. Besser, D.D. Hancock, and D.R. Call. 2006. Use of a nonmedicated dietary supplement correlates with increased prevalence of Sterptomycin-sulfa-tetracycline-resistant Escherichia coli on a dairy farm. Applied and Environmental Microbiology. 72(7):4583-4588.
Luo, H., Y. Cai, Z. Peng, T. Liu, and S. Yang. 2014. Chemical composition and in vitro evaluation of the cytotoxic and activities of supercritical carbon dioxide extracts of pitaya (Dragon fruit) peel. Chemistry Central Journal. 8(1):1-7.
Nurliyana, R., Z.I. Syed, S.K. Mustapha, M.R. Aisyah, and R.K. Kamarul. 2010. Antioxidant study of pulps and peels of dragon fruits a comparative study. Food Research Journal Malaysia. 17(2):367-375.
Nurmahani, M.M., A. Osman, A. Abdul Hamid, F.
Mohamad Ghazali, and M.S.P. Dek. 2012. Antibacterial property of Hylocereus polyrhizus and Hylocereus undatus peel extracts. Food Res Journal. 19(1):77-84.
Olaleye, M.T. 2007. Cytotoxicity and antibacterial activity of methanolic extract of Hibiscus sabdariffa. Journal of Medicinal Plants Research. 1(1):009-013.
Phebe, D., M.K. Chew, A.A. Suraini, O.M. Lai, and O.A. Janna. 2009. Red-fleshed pitaya (Hylocereus polyrhizus) fruit colour and betacyanin content depend on maturity. International Food Research Journal. 16:233-242.
Poernomo, J. 2004. Variasi tipe antigen Salmonella pullorum yang ditemukan di Indonesia dan penyebaran serotipe Salmonella pada ternak. WARTAZOA. 14(4):143-159.
Pratiwi, S.T. 2008. Mikrobiologi Farmasi. Penerbit Erlangga. Jakarta.
Putra, I.A., Erly dan M. Masri. 2015. Uji efek antibakteri ekstrak etanol kulit batang salam {Syzigium polyanthum (Wight) Walp} terhadap Staphylococcus aureus dan Escherichia coli secara invitro. Jurnal Kesehatan Andalas. 4(2):497-501.
Robinson, T. 1995. Kandungan Organik Tumbuhan Tinggi. Edisi keenam. Terjemahan Padmawinata K. Penerbit ITB : Bandung.
Seeley, H.W., P.J.V. Demark and J.J. Lee. 2001. Microbes in Action: A Laboratory Manual of Microbiology .4th Edition. W.H. Freeman and Company, New York.
Shivaprasad, H.L. 1997. Pullorum Disease and Fowl Typhoid. In: Diseases of Poultry. 10th Ed. CALNEK (Ed.). Iowa, State University Press . Ames. Iowa. USA.
Van, D.B.A.E., N. London, C. Driessen, and E.E. Stobberingh. 2001. Antibiotic resistance of faecal Escherichia coli in poultry, poultry farmers and poultry slaughterers. Journal of Antimicrobial Chemotherapy. 47(6):763-771.
Wahdaningsih, S., E.K. Untari, dan Y. Fauziah. 2014. Antibakteri fraksi n-Heksana kulit Hylocereus polyrhizus terhadap Staphylococcus epidermidis dan Propionibacterium acnes. Pharm. Sci. Res. 1(3):180-193.
Zuhud, E.A., W.P. Rahayu, C.H. Wijaya, dan P.P. Sari. 2001. Aktivitas antimikroba ekstrak kedawung (Parkia roxburghii G. Don) terhadap bakteri patogen. J. Teknologi Industri Pangan. 12:6-12.
DOI: https://doi.org/10.21157/j.med.vet..v12i1.4241
Article Metrics
Abstract view : 0 timesPDF - 0 times
Refbacks
- There are currently no refbacks.
Copyright (c) 2018 by author and J. Med. Vet.
This work is licensed under a Creative Commons Attribution 4.0 International License.
J. Med. Vet. is licensed under a Creative Commons Attribution 4.0 International License.