16. The Number Of Leukocyte And Leukocyte Differential In Broilers That Infected With Eimeria tenella And Given Neem Leaf Extract And Jaloh Extract

Rizki Aulia, Sugito Sugito, M. Hasan, T. Fadrial Karmil, Gholib Gholib, Rinidar Rinidar

Abstract


The aim of this research was to determine the effect of giving neem leaf extract combined with jaloh towards the total of leukocytes and differential leukocyte in broilers that was infected with Eimeria tenella. This research samples were 20 Cobb stain broilers 14 days old. Design the research was complete randomized design with five treatments and each treatment consists of four repetitions. First treatment (P1) as negative control which was only given mineral water; second treatment (P2) as positive control was is given 5 mg/L anti-stress commercial in drinking water; Third treatment (P3) was given 1000 mg/L Jaloh extract; Fourth treatment (P4)was given 250mg/L neem extract; and fifth treatment (P5)was given 1000mg/L jaloh extract combined with 250mg/L neem extract. Anti-stress commercial and extract treatment dissolved in drinking water. The treatment start from 08.00 until 18.00 for ten days. Next in the 11th day (chicken 25 days old)was inoculated Eimeria tanella sporulatif as much as 1 x 104 ookista/ml orally. Then the blood was taken sampling when the chicken 30 days old (five days after infected). Blood sampling was done in the brachial vein. Furthermore, the number of leukocytes and differential leukocyte.Was calculated data were analyzed using complete randomized design with the help of SPSS for Windows 1.8 program. The result of this research showed that giving jaloh and neem leaf extract were not giving significant effect (P>0,05) towards the number of leukocyte as well as differential leukocyte of broilers that was infected with Eimeria tenella. The conclusion of this research were giving jaloh leaf extract and neem for 10 days was not giving significant effect towards number of leukocytes and differential leukocyte broilers that infected with Eimeria tenella

Keywords


Broiler; Eimeria tanella; leukocyte; differential.

Full Text:

PDF

References


Ahmed, A., W.A. Shah, S. Akbar, M. Younis, and D. Kumar. 2011. A short chemical review on salix caprea commonly known as goat willow. Int. J. Res. Phytochem. Pharmacol. 1:17-20.

Asgarpanah, J. 2012. Phytopharmacology and medicinal properties of Salix aegyptiaca L. African J. Biotechnol. 11(28): 7145-7150.

Brown, B.A. 1980. Hematology: Principiles and Prosedures. 3th ed. Lea and Febriger. Philadelphia.

Cahyaningsih, U dan Supriyanto. 2007. Kejadian Koksidiosis pada Domba Umur 6-12 Bulan di Ciomas Bogor. Prosiding Seminar PERSADA XIII. Institut Pertanian Bogor, Bogor. Hal. 159

Chrubasik, S., E. Eisenberg, E. Balan, T. Weinberger, R. Luzzati, and C. Conradt. 2000. Treatment of low back pain exacerbations with willow bark extract: A randomized double-blind study. Am. J. Med. 109: 9-14.

Fabricant, D.S. and N.R Farnsworth. 2001. The value of plants used in traditional medicine for drug discovery. Environ. Health Perspect.109(1): 69–75.

Fiebich, B.L. and S. Chrubasik. 2004. Effects of an ethanolic salix extract on the release of selected inflammatory mediators in vitro. Phytomedicine.11: 135-138.

Ganong, W. 1995. Buku Ajar Fisiologi Kedokteran. (Diterjemahkan oleh M.D. Widjajakusuma). Penerbit EGC, Jakarta.

Guyton, A.C. 1995. Fisiologi Kedokteran dan Mekanisme Penyakit. Penerbit EGC, Jakarta

Guyton, A.C. 1996. Buku Ajar Fisiologi Kedokteran. Penerbit EGC, Jakarta.

Harismah, A. 2006.Pengaruh Pemberian Ekstrak Sambiloto (Androwaphi. f Panlculota ness) dengan Pelarut Air Dosis Bertingkat Terhadap Jumlah Skizon, Makrogamet, Mikrogamet dan Ookista Eimeria tenella pada Sekum Ayam. Skripsi. Fakultas Kedokteran Hewan Institut Pertanian Bogor. Bogor.

Kahkonen, M.P., A. I. Hopia, dan H.C. Fuorella. 1999. Antioxidant Activity of Extract Containing Phenolic Compound. J. Agric. Food Chem. 4(7): 3954-3962

Melvin, J.S. and O.R. William. 1993. Dukes Physiology of Domestic Animal. 11th ed. Cornel University Press. London.

Metcalf, D. 2006. Leukocyte.http://en.wikipedia.org /Leukocyte. Diakses [27 November 2014].

Mitruka, B. M and Rawnsley. H. M. 1981. Clinical Biochemical and Hematological Reference Vawes in Normal Experimental Animals. Masson. New York.

Prapanca, I dan Marianto, L.A. 2003. Khasiat dan Manfaat Sambiloto Raja Pahit Penakluk Aneka Penyakit. Agromedia Pustaka Jakarta.

Ruskin. 1993. Pestisida Nabati Ramuan Dan Aplikasi. P.T. Penebar Swadaya, Bogor.

Sastrodiharjo, S. 1998. Evaluasi Daya Insektisida dari Ekstrak Daun Mimba (Azadirzchta indica A. Juss). Seminar Hasil Penelitian Pangan dan Gizi. Ilmu Hayati, Jakarta.

Shirley, M.W. 1993. Live Vaccines for Control of Coccidiosis. Proceding: 6th International Coccidiosis Conference. New York :

McGraw Hill. Hal. 24-27

Smith, B.J. dan S. Mangkoewidjojo. 1988. Pemeliharaan, Pembiakan dan Penggunaan Hewan Percobaan di Daerah Tropis Indonesia. University Press. Jakarta.

Soulsby, E.J.L. 1982. Helminth Arthropods and Protozoa of Domesticated Animals. 7th ed. Bailliere Tindal, London.

Steel, R. and Torrie, J. 1991. Prinsip dan Prosedur Statistika Suatu Pendekatan Biometrik. PT. Gramedia Pustaka Utama, Jakarta.

Sugito, E. Rahmi, dan R. Sumarni. 2008. Penentuan dosis letal ekstrak n-heksan kulit batang jaloh (Salix tetrasperma roxb) dan efeknya terhadap perubahan histopatologi hati dan ginjal mencit. Seminar hasil-hasil Penelitian Dosen dan Mahasiswa BKS-PTN Barat, Banda Aceh.

Sukrasno dan Tim Lentera. 2003. Mengenal Lebih Dekat Mimba Tanaman Obat Multifungsi. Agromedia Pustaka. Jakarta.

Syarmalina, dan D.R. Laksmitawati, 2005. Uji Antibakteri ekstrak daun Mimba(Azadirachta indica A Juss) Terhadap Bakteri. Prosiding Seminar Nasional Tumbuhan Obat Indonesia XXVIII, Bogor.

Tampubolon, M.P. 2004. Protozoologi. Fakultas Kedokteran Hewan Institut Pertanian Bogor, Bogor.

Tizard, I. 1982. Veterinary Immunology. 3rd ed. Airlangga University Press. Surabaya.

Tizard, I.R.. 1988. Pengantar Imunologi Veteriner. Airlangga University Press, Surabaya.

Zakir, Z. 1996. Tendensi Pengobatan Coccidiosis di Masa Datang. Poultry Indonesia.




DOI: https://doi.org/10.21157/j.med.vet..v11i2.4667

Article Metrics

Abstract view : 0 times
PDF - 0 times

Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 by author and J. Med. Vet.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Creative Commons License
J. Med. Vet. is licensed under a Creative Commons Attribution 4.0 International License.