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  3. Maulana

ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF NUTMEG (Myristica fragrans Houtt) STEM BARK

Ilham Maulana, Binawati Ginting, Nurdin Nurdin, Saiful Fakri

Abstract


Antioxidant activity test for the extract of ethyl acetate nutmeg stem bark (Myristica fragrans Houtt) was carried out using 2,2-diphenyl-1picrylhydrazil (DPPH). The test results of antioxidant activity of ethyl acetate nutmeg stem bark extract with DPPH method at a concentration of 25 ppm, 50 ppm and 100 ppm obtained IC50 value = 68.14 ppm with IC50 value of vitamin C (as positive control) 3.657 ppm. The results of column chromatographic separation of ethyl acetate nutmeg stem bark extract obtained 5 combined fractions namely MFEK 1, MFEK 2, MFEK 3, MFEK 4 and MFEK 5. The test results of the fraction antioxidant activity in a good category were the MFEK 1 fraction. The MFEK 2 fraction to MFEK 5 showed an antioxidant activity lower than compared  to the ethyl acetate extract. This shows that the ethyl acetate nutmeg stem bark extract has a better inhibitory activity, because there are several active compounds contained in the extract which can inhibit free radicals, so the IC50 value of the extract is better than the fractions.


Keywords


Nutmeg stem bark (Myristica fragransHoutt), Ethyl Acetate Extract, DPPH, Antioksidant

References


Ginting, B, T. Barus, P, Simanjuntak, L. Marpaung., 2013, Isolation and Determination of Antioxidant Activity in Total Nutmeg Leaf Alkaloid (Mirystica fragrans Houtt), Proceedings of the Yusuf Banseh National Seminar.

Prakash, E and Dwijendra, K.Gupta, 2013, Cytotoxic Activity of Ethanolic Extract of Myristica fragrans (Houtt) against seven Human Cancer Cell Lines. Journal of Food and Nutrition Science. Volume 1(1), Hal. 1-3.

Dewi, K., Budi Widyarto, Pande Putu Erawijantari, Wahyu Widowati, 2015, In vitro study of Myristica fragrans seed (Nutmeg) ethanolic extract and quercetin compound as anti-inflammatory agent, International Journal of Research in Medical Sciences Dewi K et al. Int J Res Med Sci. 3(9):2303-2310 www.msjonline.org

Cao, G.Y, Xiu-Wei Yang, Wei Xu, Fei Li, 2013, New inhibitors of nitric oxide production from the seeds of Myristica fragrans, Journal of Food and Chemical Toxicology, Volume 62. Hal. 167–171.

Cao, G.Y, Wei Xu, Xiu-Wei Yang, Frank J. Gonzalez, Fei Li, 2015, New neolignans from the seeds of Myristica fragrans that inhibit nitric oxide production, Journal of Food Chemistry, Volume 173. Hal. 231–237

Acuña, U.M, Peter J. Blanco Carcache, Susan Matthew, Esperanza J. Carcache de Blanco, 2016, New acyclic bis phenylpropanoid and neolignans, from Myristica fragrans Houtt., exhibiting PARP-1 and NF-JB inhibitory effects, Journal of Food Chemistry, Volume 202. Hal. 269–275.

Hellen, M, Vargheese, T.N, Kumari, J, Abiramy, Sajina, Sree, J, 2012, Phytochemical Analysis and Anticancer Activity of Essential Oil From Myristica fragrans, International Journal of Current Pharmaceutical Review and Research.

Ginting, B., 2013, Antifungal Activity of Nutmeg Leaf Extract (Mirystica fragrans Houtt), Proceedings of the 2013 National Chemical Seminar.

Ginting, B., T. Barus, P, Simanjuntak, L. Marpaung., 2014, Isolation and Antioxidant Properties of Total Flavonoids of Leaf Nutmeg (Mirystica fragrans Houtt), Proceedings of the National Chemical Seminar, Samarinda

Akinboro, A , Kamaruzzaman, M. B, Asmawi, M. Z, Sulaiman, S. F, Sofiman, O. H, 2011, Antioxidans in Aqueous extract of Mirystica fragrans (Houtt) Suppress Mitosis and Cyclophosphamide inducechromosomal aberrations in Allium cepa L. Cells, Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology). ISSN1673-1581 (Print);ISSN18621783(Online).www.zju.edu.cn/jzus

Ginting, B, T. Barus, P, Simanjuntak, L. marpaung., 2013, Isolation and Antioxidant Properties of Total Nutmeg Leaf Alkaloid (Mirystica fragrans Houtt), Proceedings of SNYuBe

Gupta, A. D, Bansal,V. K, Babu, V, Maithil, N., 2013. Chemistry, Antioxidan and Antimicrobial Potential of Nutmeg (Myristica fragrans Houtt). Journal of Engineering and Biotecnology

Pillai S. P, Roziahanim M., Amin M. S., Zeyad D., Mahmoud N., 2012. Antioxidant and antiangiogenic activities of the essential oils of Myristica fragrans and Morinda citrifolia. Asian Pacific Journal of Tropical Medicine, Hal. 294-298

Chakraborty, P., Lavanya P. and Jayanthi A., 2015, Bioactivity of Myristica fragrans Methanol Extract, Journal of Pharmaceutical Research,Volume 4, Issue 9, 1145-1157. Research Article ISSN 2277– 7105.

Sulaiman, F. S, Ooi, L. K, 2012, Antioxidant and anti Food-Born Bacterial Activities of Extracts from Leaves and Different fruit parts of Myristica fragrans Houtt., Journal Food Control25 : 533e536. Peneng, Malaysia.

Lima K. Rafaela, Cardoso, M. G, Andrade, M.A, Guimaraes, P. L, Batista, L. R, Nelson, D. L, 2012, Bactericidal and Antioxidant Activity of Essential Oils From Mirystica fragrans Houtt and Salvia microphylla H.B.K, J Am Oil Chem Soc (2012) 89:523–528 DOI 10.1007/s11746-011-1938-1

Kazeem MI., Akanji MA., Hafizur Rahman M., Choudhary MI., 2012. Antiglycation, antioxidant and toxicological potential of polyphenol extracts of alligator pepper, ginger and nutmeg from Nigeria. Asian Pacific Journal of Tropical Biomedicine. Vol 2(9). Hal. 727-732.

Awe, F.B., T. N. Fagbemi, B. O. T. Ifesan, A. A. Badejo, Antioxidant properties of cold and hot water extracts of cocoa, Hibiscus flower extract, and ginger beverage blends, 2013, Food Research International 52, 490–49.

Ginting, B, Mustanir, Hira H., Lidya S.D., Siti H., Nurmilasari, Ummu S., 2016, Phytochemical Studies and Development of Cancer Drugs from Pala Plants (Mirystica fragrans Houtt), Ministry of Health of the Republic of Indonesia, Health Research and Development Center, Center for Research and Development.

Ramaswamy, V, N. Varghese, A.Simon,2011, An Investigation on Cytotoxic and Antioksidant Properties of Clitoria Ternatea L. International Journal of Drug Discovery. Vol.3 : 74-77. ISSN : 0975-4423.

Gessler, M. C., Nkunya, L.B. Mwasumbi, M. Heinrich and M. Toner, 1994, Screening tanzanian medical plants antimalarial activity, Acta Trop. 56:65-77.

Harborne, J.B., 1987, Metode Fitokimia: Penentuan Cara Moderen Menganalisa Tumbuhan. Terjemahan dari Phytochemical Methods oleh Kosasih Padmawinata.


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DOI: 10.24815/jn.v19i3.12618

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