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  3. SAYUTHI

Virulence of conidia Beauveria bassiana (Bals.) as a bioinsecticide against Crocidolomia pavonana (F.) (Lepidoptera: Pyralidae) on broccoli plants

MUHAMMAD SAYUTHI, ALFIAN RUSYDI, HASNAH HASNAH, NAILA EVI AZAHRA

Abstract


Crocidolomia pavonana is an important pest of broccoli plants. Pest control is currently still using chemical pesticides that negatively impact the environment. It is hoped that Beauveria bassiana will become one of the environmentally friendly bioinsecticides. The research aimed to study the virulence level of conidia density of B. bassiana as a bioinsecticide against C. pavonana pests on broccoli plants. This experiment used a completely randomized design consisting of 5 treatments and four replications so that 20 experimental units were obtained. The Basic Plant Protection Laboratory and Plant Pest Science Laboratory Faculty of Agriculture, Syiah Kuala University as the research location, which was carried out from January to September 2021. The parameters observed were larval mortality, pupae formed, imago appeared, an incubation period of B. bassiana conidia, and average time of C. pavonana. The results showed that B. bassiana with conidia density of 108/mL distilled water was effective as a bioinsecticide against C. pavonana. The highest percentage of C. pavonana mortality observed at 6 Days After Application (DAA) occurred at a density of 108 conidia/mL of water (97.50%), and the lowest was at a density of 102 conidia/mL of distilled water (62.50%). The percentage of pupae formed was observed. At 7, DAA was 15% using a density of 102 conidia /mL aquadest. Produce 5%, a density of 104 conidia/mL of distilled water was used. The percentage of imago that appeared with a conidia density of 102/mL of distilled water killed at 14 DAA yielded 15%, and 5% resulted from a density of 104 conidia/mL of distilled water. The fastest incubation occurred at a density of 108 conidia/mL of distilled water with a time of 3.50 days. Death of C. pavonana at 3.03 days required conidia density of B. bassiana 102/mL of distilled water.


Keywords


Beauveria bassiana, Crocidolomia pavonana, pest, Insecticide

References


REFERENCE

Abbott, W.S. 1925. A method of computing the effectiveness of an insecticide. J. Economic Entomology. 18:265:267

Ambarawati, N. 2012. The effectiveness of wood vinegar as a vegetable pesticide in controlling Crocidolomia pavonana and plant stimulants in mustard greens. Essay. Sebelas Maret University Faculty of Agriculture Study Program. Surakarta.

Anggarawati, S.H.; T. Santoso. and Anwar, R. 2017. Use of entomopathogenic fungi Beauveria Bassiana (Balsamo) Vuillemin and Lecanicillium Lecanii (Zimm) Zare & Gams to control Helopeltis Antonii Sign (Hemiptera: Miridae). J. Tropical Silviculture. 8(3): 199

Aror, A.P.F.; Rante, C.S. and want.N,N. 2017. Utilization of the entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillemin against Plutella xylostella (L.) larvae in the laboratory. Research article. Agrotechnology Study Program. Sam Ratulangi University. Manado.

Budi, A.S.; Afandhi.A and Puspitarini. R.D. 2013. Pathogenicity of Entomopathogenic Fungus Beauveria bassiana Balsamo (Deuteromycetes: Moniliales) on Larvae of Spodoptera litura Fabricius (Lepidoptera: Noctuidae). J. HPT 1(1): 57-65.

Gabriel, B.P and Riyanto. 1989. Metarhizium anisopliae Taxonomy, Pathology, and Applications. Plantation Crop Protection Development Project. Plantation Crop Protection Directorate. Agriculture department. Jakarta. 25 Pages.

Gomez, K and Gomez.A.A, 1995. Statistical Procedures for Agricultural Research, 2nd Edition. Publisher University of Indonesia (UI-Press): Jakarta.

Hasibuan, R.;Levilia, H.; Wibowo, L and Purnomo. 2013. Growth of the fungus Beauveria bassiana (Bals) Vuill and its pathogenicity to soybean aphids (Aphis glycines Matsumura). J. Tropical Agrotech. 1(3): 283-288.

Herminanto. 2006. Control of cabbage pests Crocidolomia pavonana F using orange peel extract. J. Rural Development. 6(3): 166-174.

Humairoh, D.M.; Hidayat.T.; Isnawati and Prayogo.Y. 2013. Effect of combination of entomopathogenic fungi and conidia density on the intensity of attack by armyworm larvae. J. Bio Lantern. 2(1): 19-23.

Hughes, S.J. 1971. Phycomycetes, basidiomycetes, and ascomycetes as fungi imperfecti. in: taxonomy of fungi imperfecti. University of Toronto Press, Toronto.

Kaur, S.; Kaur.H.P; Kaur.K and Kaur.A. 2011. Effect of different concentrations of Beauveria bassiana on development and reproductive potential of Spodoptera litura (Fabricius). J. Biopest. 4(2): 161-168.

Ladja, F.T.; Santoso. T; Nurhayati.E. 2011. Potential of entomopathogenic fungi Verticillium lecani and Beauveria bassiana in controlling green leafhoppers and suppressing Tungro disease intensity. J. Food Crops Agricultural Research. 30(2): 114-120.

Permadi, M.A.; Anwar,R and Santoso.T. 2017. Utilization of the fungus Beauveria bassiana (Bals.) Vuill. as a mico-insecticide against the citrus hopper Diaphorina citri Kuw. (hemiptera: liviidae). J. Environmental Biology, Industry, Health. 4(1): 82-89.

Pertiwi, S.P.; Hasibuan.R and Wibowo.L. 2016. The effect of the entomopathogenic fungus Beauveria bassiana on spore growth and mortality of soybean aphids (Aphis glycines Matsumura). J. Tropical Agrotech. 4(1): 55-61.

Rosmiati, A.; Hidayat.; Firmansyah.E and Setiati.Y. 2018. The potential of Beauveria bassiana as a biological agent of Spodoptera litura Fabr. on soybean plants. J. Agriculture. 29(1):43-47.

Salbiah, D.; Laoh, J.H. and Nurmayani. 2013. Test multiple doses of Beauveria bassiana Vuillmen against larvae of the horn beetle Oryctes rhinoceros (Coleoptera: Scarabaeidae) in oil palm. J. Scientific Applied Science. 4(2): 173-142.

Sari, N. J and Prijono. D. 2004. Development and reproduction of Crocidolomia pavonana (F.) (Lepidoptera: Pyralidae) in natural and semi-manufactured feeds. J. Pests and Diseases of Tropical Plants. 4(2): 53-61.

Sayuthi, M.; Santoso,T and Harahap.I.S. 2011. Symptomatology and time of death of termites Macrotermes gilvus Hagen (isoptera: family termitidae) after infection with the fungus Metarhizium brunneum Petch. J. Biology News. 10(6): 729-736.

Sianturi, N.B.; Pangestiningsih.Y and Lubis.L. 2014. Test the effectiveness of the entomopathogenic fungi Beauveria bassiana (Bals.) and Metarrhizium anisopliae (Metch) against Chilo sacchariphagus Boj. (Lepidoptera: Pyralidae) in the Laboratory. J. Online Agrotechnology. 2(4): 1607-1613.

Soetopo, D and Indrayani. I. 2007. Technological status and prospects of Beauveria bassiana for environmentally friendly insect pest control of plantation crops. perspective. J. Institute for tobacco and fiber research. 6(1): 29-46.

Sritamine, A.A.G. 2017. Effect of several types of weed leaf extract on the biology of the cabbage head caterpillar (Crocidolomia pavonana F.) in the laboratory. J. Tropical Agroecotechnology. 6(4): 370-377.

Surtikanti and Yasin.M. 2009. The entomopathogenic effectiveness of Beauveria bassiana Vuill. from various growing media against Spodoptera litura F. (Lepidoptera: Noctuidae) in the laboratory. Cereal plant research institute. Proceedings of the serelia national seminar.

Tantawizal. ; Inayati. A and Prayogo.Y. 2015. Potential of the Entomopathogenic Fungus Beauveria bassiana (Balsamo) Vuillemin to Control Boleng Cylas formacarius F. Pests on Sweet Potato Plants. Research Center for Various Nuts and Tubers. No. 29: 46-53.

Talib, R.; Fernando.R.; Khodijah. ; Meidalima. D and Herlinda.S. 2013. Pathogenicity of isolates of Beauveria bassiana and Metarhizium anisopliae from Lebak and tidal soils of South Sumatra for the biological agent Scirpophaga incertulas. J. Tropical HPT. 13(1): 10-18.

Trizelia. 2005. The entomopathogenic fungus Beauveria bassiana (Bals.) Vuill. (deuteromycotina: hyphomycetes): genetic diversity, physiological characterization, and virulence against Crocidolomia pavonana (F.) (Lepodiptera: Pyralidae). Essay. Bogor Agricultural Institute. Bogor.

Trizelia.; T. Santoso.; Sosromarsono.S ; Rauf.A ; Sudirman.L.I 2012. Genetic diversity of various isolates of Beauveria bassiana (Bals.) Vuill. (Deuteromycotina: Hyphomycetes) and their virulence against Crocidolomia pavonana. J. Nature Indonesia. 14(3): 176-183.

Wicaksono, A.P.; Abadi. A.L. and fandi.A. 2015. Test the effectiveness of the application method of the entomopathogenic fungus Beauveria Bassiana (Bals.) Vuillemin against the pupaee of Bactrocera carambolae Drew & Hancock (Diptera: Tephritidae). J. HPT. 3(2): 39-49.

Wibawanti, R and Herminanto. 2010. Potential of entomopathogenic fungus Beauveria bassiana Vuill to control armyworm (Spodoptera litura F.). J. Rural Development 10(1): 44-45.


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DOI: 10.24815/jn.v22i1.22628

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