The effect of plant growth promoting Rhizobacteria treatment on germination and seedlings growth of chilli

SYAMSUDDIN SYAMSUDDIN, SITI HAFSAH, VINA MAULIDIA, AINUN MARLIAH

Abstract


The aim of the research was to study the effects of biological seed treatment utilization rhizobacteria on seed germination and seedling growth, furthermore to figure out the competence of rhizobacteria isolates to dissolve phosphate, produce acetic indol acid (IAA) and siderophore. 18 rhizobacteria isolates used in this research, each isolates was anlayze in vitro; IAA, phosphate and siderophore. Then carried out the in vitro test, the pepper seeds were inoculated with rhizobacteria isolates suspension then germinated. Seedlings were transpalated into a plastic pot filled with medium soil and compost (2:1). The germination was observed everyday until 14 days, the seedlings was observed at 4 and 6 weeks after transplating. Based on the study, all the rhizobacteria isolates produced IAA, 13 rhizobacteria isolates capable to dissolve phosphate and 12 rhizobacteria isolates produced siderophore. Seed treatment using RBNA 14, RBNA 13, RBKB 5, and RBSPA 14 adequate to increase the germination in seed viability and vigor also increase seedling growth

Keywords


isolate, IAA, phospate, siderophore, transplanting

References


BPS (Badan Pusat Statistik). 2020. Horticulte Statistic 2020. https://www. bps.go.id/publication/2021/06/07/daeb50a95e860581b20a2ec9/statistikhortikultura-2020.html

Bhat, M, A.; Kumar, V.; Bhat, M, A.; Wani, I, A.; Dar, F, L.; Farooq, I.; Bhatti, F.; Koser, R.; Rahman, S.; Jan, A, T. 2020. Mechanistic Insights of the Interaction of Plant Growth Promoting Rhizobacteria (PGPR) With Plant Roots Toward Enhancing Plant Productivity by Alleviating Salinity Stress. Front Microbiol. 11 1952. doi:10.3389/fmicb. 2020.01952

Egamberdiyeva, D. 2008. Plant growth promoting properties of rhizobacteria isolated from wheat and pea grown in loamys and soil. Turk. J. Biol. 32 9–15

Bae, Y, S.; Park, K, S.; Lee, Y, G.; Choi, O, H. 2007. A simple and rapid methode for functional analysis of plant growth-promoting rhizobacteria using the development of cucumber adventious root system. Plant Pathol. J. 23 223-225.

Danish, S.; Zafar, M.; Mohsin, F.; Hussain, M. 2020. ACC-deaminase producing plant growth promoting rhizobacteria and biochar mitigate adverse effects of drought stress on maize growth. PLoS One. 15:e0230615.doi: 10.1371/journal.po ne.0230615

Dixit, V, K.; Misra, S.; Mishra, S, K.; Tewari, S, K.; Joshi, N.; Chauhan, P, S. 2020. Characterization of plant growth-promoting alkalotolerant Alcaligenes and Bacillus strains for mitigating the alkaline stress in Zea mays. Ant. Van Leeuwenhoek. 113 889–905.doi: 10.1007/s10482-020-01399-1

Li, H, Q.; and Jiang, X, W. 2017. Inoculation with plant growth-promoting bacteria (PGPB) improves salt tolerance of maize seedling. Russ. J. Plant Physiol. 64 235–241.doi: 10.1134/s102144371702 0078

You, M.; Fang, S.; MacDonald, J.; Xu, J.; Yuan, Z, C. 2020. Isolation and characterization of Burkholderia cenocepacia CR318, a phosphate solubilizing bacterium promoting corn growth. Microbiol. Res. 233126395.doi: 10.1016/j. micres.2019.126395

Ali, S.; Hameed, S.; Shahid, M.; Iqbal, M.; Lazarovits, G.; Imran, A. 2020. Functional characterization of potential PGPR exhibiting broad-spectrum antifungal activity. Microbiol. Res. 232 126389.doi: 10.1016/j.micres.2019.1263 89

Li, Y.; Zeng, J.; Wang, S.; Lin, Q.; Ruan, D.; Chi, H. 2020. Effects of cadmium-resistant plant growth-promoting rhizobacteria and Funneliformis mosseae on the cadmium tolerance of tomato (Lycopersicon esculentum L.). Int. J. Phytoremediat. 22 451–458.doi: 10.1080/ 15226514.2019.1671796

Chandra, D.; Srivastava, R.; Gupta, V, V, S, R.; Franco, C, M, M.; Sharma, A, K. 2019. Evaluation of ACC-deaminase-producing rhizobacteria to alleviate water-stress impacts in wheat (Triticum aestivum L.) plants. Can. J. Microbiol. 65 387–403.doi: 10.1139/cjm-2018-0636

Rima, F, S.; Biswas, S.; Sarker, P, K.; Islam, M, R.; Seraj, Z, I. 2018. Bacteria endemic to saline coastal belt and their ability to mitigate the effects of salt stress on rice growth and yields. Ann. Microbiol. 68 525–535.doi: 10.1007/s132 13018-1358-7

El-Esawi, M, A.; Alaraidh, I, A.; Alsahli, A, A.; Alzahrani, S, A.; Ali, H, M.; Alayafi, A, A. 2018. Serratia liquefaciens KM4 improves salt stress tolerance in maize by regulating redox potential, ion homeostasis, leaf gas exchange and stress-related gene expression. Int. J. Mol. Sci. 19 3310.doi: 10.3390/ijms1911 3310

Fukami, J.; de la Osa, C.; Ollero, F, J.; Megías, M.; Hungria, M. 2018. Coinoculation of maize with Azospirillum brasilense and Rhizobium tropici as a strategy to mitigate salinity stress. Funct. Plant Biol. 45 328–339.

Akram, M, S.; Shahid, M.; Tariq, M.; Azeem, M.; Javed, M, T.; Saleem, S. 2016. Deciphering Staphylococcus sciuri SAT-17 mediated anti-oxidative defense mechanisms and growth modulations in salt stressed maize (Zea mays L.). Front. Microbiol. 7 867.

Adesemoye, A, O.; Obin, M.; Ugoji, E, O. 2008. Comparison of plant growth-promoting with Pseudomonas aeruginosa and Bacillus subdtilis in three vegetable. Brazilian J. of Microbiol. 39 423-426.

Raj, S, N.; Shetty, H, S.; Reddy, M, S. 2005. Plant growt promoting rhizobacteria: Potential green alternative for plant productivity. ZA Siddiqui (ed.), PGPR: biocontrol and biofertilization, 1 197-216. Springer. Netherlands.

Faccini, G.; Garzon, S.; Martines, M.; Varela, A. 2004. Evaluation of the effects of a dual inoculum of phosphate-solubilizing bacteria and Azotobacter chroococcum, in creollo potato (papa “Criolla”) (Solanum phureya) var ‘Yema de Huevo’. J. auburn edu argent. 1 1-9

Kumar, R, S.; Ayyadurai, N.; Pandiraja, P.; Reddy, A, V.; Venkateswarlu, Y.; Prakash, O.; Sakthivel, N. 2005. Characterization of antifungal metabolite produced by a new strain Pseudomonas aeruginosa PUPa3 that exibits broad-spectrum antifungal activity and biofertilizing traits. J. of App. Microbiol. 98 145-154.

Konappa, N.; Soumya, K.; Udayashankar, C, A.; Srivinas, C. 2020. Efficacy of indigenous plant growth-promoting rhizobacteria and Trichoderma strains in eliciting resistance against bacterial wilt in a tomato. Egyp. j. of biol. pest cont. 30 1-9. DOI:10.1186/s41938-020-00303-3

Yanti, Y.; Astuti, F, F.; Habazar, T.; Nasution, C, R. 2017. Screening of rhizobacteria from rhizosphere of healthy chili to control bacterial wilt disease and to promote growth and yield of chili. Biodiversitas. 17 1-9.

Gao, B.; Zhang, X.; Tian, C.; Zhang, X.; Liu, J. 2020. Effects of amendments and aided phytostabilization of an energy crop on the metal availability and leaching in mine tailings using a pot test. Environ. Sci. Pollut. Res. 27 2745e2759. https://doi.org/10.1007/s11356-019-0717 1-x.

Glickman, E.; Dessaux, Y. 1995. A critical examination of specificity of the salkowski reagent for indolic compound by phytopathogenic bacteria. Appl. Environ. Microbiol. 61 793- 796.

Goldstein, A, H. 1986. Bacterial solubilization of mineral phosphates. Historical perspective and future prospects. Amer. J. Alternat. 1 1-9

Dell'Amico, E.; Cavalca, L.; Andreoni, V. 2008. Improvement of Brassica napusgrowth under cadmium stress by cadmium-resistant rhizobacteria. Soil Biol. Biochem. 40 74–84. 10.1016/j. soilbio. 2007.06.024

Bai, Y.; Pan, B.; Charles, T, C.; Smith, D, L. 2002. Co-inoculation dose and root zone temperatur vfor plant growth promoting rhizobacteria on soybean (Glycine max L. Merr) grown in soil less media. Soil Biol. Biochem. 34 1953-1957.

Copeland, L, O.; McDonald, M, B. 1995. Principles of Seed Science and Technology. Third Edition. Chapman and Hall, New York.

Thakuria, D.; Talukdar, N, C.; Goswami, C.; Hazarika, S.; Boro, R, C.; Khan, M, R. 2004. Characterization and screening of bacteria from rhizospheer of rice grown in acidic soil of Assam. Curr Sci. 86 978-985.

de Weger, L, A.; van der Bij, A, J.; Dekkers, L, J.; Simons, M.; Wijfffelman, C, A.; Lugtenberg, B, J, J. 1995. Colonization of the rhizosphere of crop plants by plant-beneficial pseudomonads. FEMS Microbiol. Ecology. 17 221-228.

Marilley, L.; Aragno, M. 1999. Phytogenetic diversity of bacterial communities differing in degree of proximity of Lolium perenne and Trifolium repens roots. App. Soil Ecology. 13 127-136.

Vansuyt, G, A.; Robin, J, F.; Briat, C, C.; Lemanceau, C, P. 2007. Iron acquisition from Fepyoverdine by Arabidopsis thaliana. Mol. Plant Microbe Int. 20 441-4417.

Chincholkar, S, B, B, L.; Chaudhari, M, R.; Rane, P, D.; Sarode. 2007. Fungi phytopathogen suppression using siderophoregenic bio-inoculants. In: Chinkolar, S.B. & K.G. Mukerji (eds.). Biological Control Of Plant Diseases: Curr. Concepts. Haworth Press. USA. 1 401-417.

Bennet, M, A. 2002. Application of biologicals to enhance vegetable seed production and quality. J. Seeds: Trade, Prod. and Techn. 1 1-9 www.faif.pup.cl/ biblioteca/articulosseminariosemillas/articu-lo09.pdf.

Nelson, L, M. 2004. Plant growth-promoting rhizobacteria (PGPR): prospects for new inoculants. Crop Management. 1 1-9 doi:101094/Cm-2004-0301-05-RV.

Hyder, S.; Amjad, S, G.; Zarrin, F, R.; Raees, A.; Muhammad, M, A.; Abdul, H.; Waqas, A.; Nida, F, M.; Inam, U, H. 2020. Characterization of native plant growth promoting rhizobacteria and their anti-oomycete potential against Phytophthora capsici affecting chilli pepper (Capsicum annum L.). Scie. RepoRtS. 10 13859 https://doi.org/10. 1038/s41598-020-694 10-31

Zakia, A.; Satriyas, I.; Candra, B.; Syamsuddin, Dyah, M. 2017. Improve plant growth of chilli and control Phytophthora blight with biopriming of seed using rhizobacteria from chili plants in east Java. J. Hort. Indones. 8 171-182.

Thilagam, R.; Hemalatha, N. 2019. Plant Growth Promotion and Chilli Anthracnose Disease Suppression Ability of Rhizosphere Soil Actinobacteria. J. of App. Microbiol. 126 1835-1849.

Jayapala, N.; Navya, H, M.; Hariprasad, P.; Hithamani, G.; Niranjana, S, R. 2019. Rhizobacteria Bacillus spp. Induce resistance against anthracnose disease in chili (Capsicum annuum L.) through activating host defense response. Egypt. J. of Biol. Pest Cont. 29 1-9. https://doi. org/10.1186/s41938-019-148-2

Gowtham, H, G.; Murali, M.; Brijesh, S, S.; Lakshmeesha, T, R.; Narasimha, M, K.; Amruthesh, K, N.; Niranjana, S, R. 2018. Plant growth promoting rhizobacteria- Bacillus amyloliquefaciens improves plant growth and induces resistance in chilli against anthracnose disease. Biol. Cont. 126 209–217. https://doi.org/10.1016/j.biocontrol.2018.05.022

Kaymak, H, C.; Yarali, F.; Guvenc, I.; Donmez, M. 2008. The effect of inoculation with plant growth rhizobacteria (PGPR) on root formation of mint (mentha piperita L.) cuttings. African J. of Biotech. 7 4479-4483.

Patten, C, L.; Glick, B, R. 2002. Role of Psemlowonas putida indoleacetic acid in development of the host plant root system. App. and Env. Microbiol. 68 3795-3801.

Ramamoorthy, V.; Samiyappan, R. 2001. Induction of defense related genes in Pseudomonas fluorescens treated chili plants in response to infection by Colletotrichumcapsici. J. Mycol. Plant Patho.l 31 146-155.

Hameeda, B.; Harini, G.; Rupela, O, P.; Wani, S, P.; Reddy, G. 2008. Growth promotion of maize by phosphate-solubilizing bacteria isolated from composts and macrofauna. J. Microbiol. Res. 163 234-242. DOI: 10.1016/j.micres. 2006.05.009

Sharf, W.; Arshad, J; Amna, S.; Iqra, H, K. 2021 Induction of resistance in chili against Sclerotium rolfsii by plant-growth-promoting rhizobacteria and Anagallis arvensis. Egypt. J. of Biol. Pest Cont. 31 1-12. https://doi.org/10. 1186/s41938-021-0036 4-y

Constantia, J.; Rejeki, S, F. 2018. Vegetative growth of rainbow chili (Capsicum annuum L.) IN the treatment of PGPR (plant growth promoting rhizobacteria), PGPR-NPK fertilizer, and PGPR-compost combination. AGRIC. 32 95-104. e-ISSN 2549-9343

Pal, A, K.; Chakraborty, A.; Sengupta, C. 2018. Differential effects of plant growth promoting rhizobacteria on chilli (Capsicum annuum L.) seedling under cadmium and lead stress. Plant Scie. Today. 5 182-190. https://dx.doi.org/10. 14719/pst.2018.5.4.419

Syamsuddin, Marlina, Tjut, C.; Vina, M. 2021. Indigenous rhizobacteria treatment in controlling diseases Phytophthora palmivora and increasing the viability and growth of cocoa seedling. Natural J. 21 105-113. doi 10.24815 / jn.v21i2.21216

Syamsuddin, Halimursyadah, Samingan, V, Maulidia. 2022. Seed treatment using rhizobacteria as plant growth promotion of two chili variety (Capsicum annuum L.). IOP Conf. Ser.: Earth Environ. Sci. 951 012060 : 1-8. doi:10.1088/1755-1315/951/1/012060


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DOI: 10.24815/jn.v22i2.24458

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