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  3. TARIGAN

Utilization of shallot extract and administration of indole acetic acid to Chrysanthemum sp in vitro culture

DAFNI MAWAR TARIGAN, WAN ARFIANI BARUS, FEMIL YANDA HAKIM NASUTION, ANGGRIA LESTAMI

Abstract


Chrysanthemum sp is a plant that has the potential to be developed in Indonesia, apart from being an ornamental flower, it can also be used as a herbal medicine. One of the efforts to develop chrysanthemum can be done in vitro by using organic growth regulators such as shallot extract and indole acetic acid (IAA). The research aims to identify the effect of shallot extract and IAA on the growth of Chrysanthemum sp. The research was conducted at UPT Central Horticulture Seed Center, Medan Johor, in December 2019 - January 2020. The research used a factorial Completely Randomized Design (CRD) with 2 factors, namely the first factor was shallot extract which consisted of 4 levels, namely 0, 35, 70, and 105 g/L water. The second factor was IAA which consisted of 4 levels, namely 0.6, 0.9, 1.2, and 1.5 mg/L water. The results showed that the administration of shallot extract had no significant effect on the growth of chrysanthemum, but the administration of IAA had a significant effect on the number of leaves and plant height with the best concentration at 0.6 mg/L water. The interaction of the two treatments also had no significant effect on the growth of chrysanthemum.

Keywords


Chrysanthemum sp, shallot extract, indole acetic acid, in vitro

References


Mustakim, W.B.F. dan Al-Fauzy. 2015. Pengaruh penambahan air kelapa terhadap pertumbuhan stek mikro tanaman krisan (Chrysantemum indicum) secara in vitro. Prosiding seminar nasional mikrobiologi kesehatan dan lingkungan. ISBN 978-602-72245-0-6. UIN Alauddin Makasar.

Badan Pusat Statistik Indonesia. 2010. Produksi Tanaman Florikultura (Hias). Jakarta.

Rahayu, M. dan Prayogi, H.E. 2013. Penambahan bahan organik pada media pertumbuhan krisan (Crysanthemum grandiflora tzvelve) secara in vitro. J. Bul.Agrohorti. 1 (4) 94-100.

Dixon, R.A. 1985. Plant Cell Culture a Pratical Approach, IRL, Press, Limited, Oxford, Washington DC.

Kumar, P.P and Loh, C.S. 2012. Plant tissue culture for biotechnology. In Chapter Book: Plant Biotechnology and Agriculture. Academic press. p. 131-138.

Singh, V; Patel, R; Kumar, S; Sahu, M.P. and Ahirwal, A. 2021. Plant growth regulators and their use in plant growth and development. Agric. Environ. 2 (4) 26-28.

Kumar, S; Singh, R; Kalia, S; Andrajwant, S.K.S and Kalia, K. 2016. Recent advances in understanding the role of growth regulators in plant growth and development in vitro – I. Convinental growth regulators. Indian Forest 142 (5) 459-470.

Alimudin, M; Syamsiah dan Ramli. 2017. Aplikasi pemberian ekstrak bawang merah (Allium cepa L.) terhadap pertumbuhan akar stek batang bawah mawar (Rosa sp.) varietas malltic. J. Agrosci. 7 (1), 194-202.

Anggara, B. dan Yuliani, S.L.L. 2014. Isolasi dan karakterisasi bakteri endofit penghasil hormon indole acetic acid dari akar tanaman ubi jalar. Lentera Bio. 3 (3),160-167.

Sumardi. 1996. Penggunaan arang aktif pada beberapa komposisi NAA dan BAP dalam kultur durian (Durio zibethinus Murr.) secara in vitro. Thesis. Program Pascasarjana Universitas Andalas, pp.76.

Rahmi, I; Suliansyah, I dan Bustamam, T. 2010. Pengaruh pemberian beberapa konsentrasi BAP dan NAA terhadap multiplikasi tunas pucuk jeruk kanci (Citrus sp.) secara in vitro. Jerami. 3 (3) 210-219.

Pasetriyani, E. T. 2014. Pengaruh macam media tanam dan zat pengatur tumbuh growtone terhadap pertumbuhan stek batang tanaman jarak pagar (Jatropa curcas Linn.). J. Agrosci. 7 82-88.

Sen, M.K; Nasrin, S; Rahman, S and Jamal, A.H. 2014. In vitro callus induction and plantlet regeneration of Achyranthes aspera L., a high value medicinal plant. Asian Pac. J. Trop. Biomed. 4 (1) 40-46.

Campbell, N.A; Reece, J.B. dan Mitchell, L.G. 2002. Biologi (Terjemahan Wasmen Manalu). Erlangga. Jakarta.

Wahyuni, D.W; Andriani, P; Ansori, A.N.M and Utami ESW. 2017. Callus induction of gendarussa (Justicia gendarussa) by various concentration of 2,4-D, IBA, and BAP. Biosaintifika 9 (3) 402-408.

Siswanto, U., N. D. Sekta dan A. Romeida. 2010. Penggunaan auksin dan sitokinin alami pada pertumbuhan bibit lada panjang (Piper retrofractum L.). Tumbuhan Obat Indonesia. 3 (2), 128-132.

Yunita, R. 2011. Pengaruh pemberian urine sapi, air kelapa dan rootone F terhadap pertumbuhan setek tanaman Markisa (Passiflora edulis var flavicarpa). Solok, 1-10.

Barus, W.A; Khair, H dan Siregar, M.A. 2014. Respon pertumbuhan dan produksi kacang hijau (Phaseolus radiatus L.) akibat pengguanaan pupuk organik cair dan pupuk TSP. Agrium. 19 (1) 1-11.

Novita, A; Cemda, A.R and Julia, H. 2017. Effects of plant hormones interaction under salt stress on growth of Roselle (Hibiscus Sabdarifa L.). ICoSAaNRM. ISBN 978-602-6997-70-8.

Rajan, R and Singh, G. 2021. A review on the use of organic rooting substances for propagation of horticulture crops. Plant Archives. 21 (1) 685-692.


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DOI: 10.24815/jn.v22i2.25778

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