Genetic diversity of Ulva lactuca from the intertidal zone in Ulee Lheue beach Aceh, Indonesia

RAHMAH HAYATI, FAWWA RAHLY

Abstract


Ulee Lheue Beach in Banda Aceh was developed for tourism. The macroalgae and seaweed species found in this area have been widespread but never exploited. Among these species is the green seaweed, Ulva lactuca, commonly known as sea lettuce. The physiological variations and genetic features of the seaweed can be attributed to the variations in the coastal environment. This study aims to describe the molecular identity of the genetic diversity of U. lactuca from the intertidal zone at Ulee Lheue Beach. The development of molecular biotechnology has enabled identification the gene expression through genomic DNA to PCR amplification. Genetic distance was determined using UPGMA. The results from 600-bp fragments were analyze the genetic diversity. A total of 15 (31%) expression identified as polymorphic (>0.500). Heterozygosity (He) and allelic differential (Na) diversity were found of 1.500–3.000. The highest PIC was observed in the rbcL1, with a correlation between subpopulations of 0.459. PCR amplification using the degenerate primer rbcL1 produced fragments ranging from 300 to 460 bp, whereas the expression of UL2 was detected at 448 and 500 bp using the rbcL2 primer. The phylogenetic identify are shown two (2) clusters. The populations of UL1, UL3, and UL4 were found a close relationship. Furthermore, the UL1 and UL2 populations were further divided into distinct clusters but related to the main branch of UL3. Based on this research, the concern of U. lactuca species for industrial and biotechnology destinations, we can describe a suitable method for obtaining he genetic distances between species.


Keywords


Beach, DNA, Genes, Ulva lactuca, Ulee Lheue

References


Handayani, S.; Widhiono, I.; Widyartini, D. S. 2023. Macroalgae diversity in the Pari Island Cluster, Seribu Islands District, Jakarta, Indonesia. Biodiversitas. 24 1659-1667. DOI: 10.13057/biodiv/d240339.

Akbar, S. A.; Hasan, M.; Afriani, S.; Nuzlia, C. 2023. Evaluation of phytochemical composition and metabolite profiling of macroalgae Caulerpa taxifolia and C. peltata from the Banda Aceh coast, Indonesia. Biodiversitas. 24 5283-5292. DOI: 10.13057/biodiv/d241009.

Dominguez, H.; Loret, E. P. 2019. Ulva lactuca, a source of troubles and potential riches. Mar. Drugs. 17 357. DOI: 10.3390/md17060357.

Gazali, M.; Effendi, I.; Wahyuni, S.; Kurniawan, R.; Matin, A.; Syafitri, R. 2023. Boosting the growth of tilapia fish using marine macroalgae (Sargassum sp.) extracts. Aquacult. Conserv. Legis. 16 1626-1635.

Hudaifah, I.; Mutamimah, D.; Utami, U. A. 2020. Komponen bioaktif dari Euchema cottonii, Ulva lactuca, Halimeda opuntia, dan Padina australis. J. Lemuru. 2 63-70. DOI: 10.36526/lemuru.v2i2.1268.

Hayati, R.; Basyuni M.; Chalil, D. 2020. Genetic diversity, sequence and bioinformatic analysis of Ganoderma boninense isolates. Int. J. Agric. Biol. 23 763-770. DOI: 10.17957/IJAB/15.1350.

Farhan, A. M.; Hanifan, A. Z; Ismi R.; Fikriyani, A.; Maulita, C. A.; Rieuwpassa. 2022. Potential extract of green algae (Ulva lactuca) as antimicrobial in mouthwash: literature review. Dental J. 11 270-274. DOI: 10.35856/mdj.v11i3.640.

Harnelly, E.; Tauhida, N.; Siregar, I. Z. 2022. Comparison of two DNA extraction methods for dry Leaf Dipterocarpaceae. J. Nat. 22 168-172. DOI: 10.24815/jn.v22i3.26588.

Wal, V. D.; Sperber, B. L.; Houweling-Tan, B.; Bakker, R. R.; Brandenburg, W.; López-Contreras, A. M. 2013. Production of acetone, butanol, and ethanol from biomass of the green seaweed Ulva lactuca. Biores. Technol. 28 431-437. DOI: 10.1016/j.biortech.2012.10.094.

Hayati, R.; Basyuni, M.; Afandi, D.; Arifianto, D.; Bimantara, Y.; Syahputra, I. 2019. Isolation and amplification DNA of Elaeis guineensis from Bangun Bandar Plantation, North Sumatra. IOP Conf. Ser.: Earth and Environ. Sci. 305 012039. DOI: 10.1088/1755-1315/305/1/012039.

Hayati, R.; Lisnawita; Munir, E.; Basyuni, M. 2020. MADS-box genes expression in seedlings of Elaeis guineensis Jacq. resistance to Ganoderma boninense. Biodiversitas. 21 5805-5810. DOI: 10.13057/biodiv/d211243.

Hulce, D.; Li, X.; Snyder-Leiby, T.; Liu, C. J. 2011. GeneMarker® genotyping software: Tools to increase the statistical power of DNA fragment analysis. J. Biomol. Tech.: JBT. 22 35-36.

Zhao,; J., Jiang, P.; Qin, S.; Liu, X.; Liu, Z.; Lin, H.; Wu, C. 2015. Genetic analyses of floating Ulva prolifera in the Yellow Sea suggest a unique ecotype. Estuarine, Coastal Shelf Sci. 163 96-102. DOI: 10.1016/j.ecss.2015.05.027.

Liu, J.; Zhao, X.; Kang, X.; Zhuang, M.; Ding, X.; Zhao, L.; He, P. 2020. Good news: we can identify Ulva species erupted in the Yellow Sea more easily and cheaply now. Conserv. Genet. Res. 12 447-449. DOI: 10.1007/s12686-019-01114-x.

Zhang, Q. C.; Yu, R. C.; Chen, Z. F.; Qiu, L. M.; Wang, Y. F.; Kong, F. Z.; Zhou, M. J. 2018. Genetic evidence in tracking the origin of Ulva prolifera blooms in the Yellow Sea, China. Algae. 78 86-94. DOI: 10.1016/j.hal.2018.08.002.

Steinhagen, S.; Karez, R.; Weinberger, F. 2019. Cryptic, alien and lost species: molecular diversity of Ulva sensu lato along the German coasts of the North and Baltic Seas. J. Phycol. 54 466-483. DOI: 10.1080/09670262.2019.1597925.

Melton, J. T.; Lopez‐Bautista, J. M. 2021. Diversity of the green macroalgal genus Ulva (Ulvophyceae, Chlorophyta) from the east and gulf coast of the United States based on molecular data. J. Phycol. 57 551-568. DOI: 10.1111/jpy.13120.

Hughey, J. R.; Maggs, C. A.; Mineur, F.; Jarvis, C.; Miller, K. A.; Shabaka, S. H.; Gabrielson, P. W. 2019. Genetic analysis of the Linnaean Ulva lactuca (Ulvales, Chlorophyta) holotype and related type specimens reveals name misapplications, unexpected origins, and new synonymies. J. Phycol. 55 503-508. DOI: 10.1111/jpy.12860.

Fort, A.; McHale, M.; Cascella, K.; Potin, P.; Usadel, B.; Guiry, M. D.; Sulpice, R. 2021. Foliose Ulva species show considerable inter‐specific genetic diversity, low intra‐specific genetic variation, and the rare occurrence of inter‐specific hybrids in the wild. J. Phycol. 57 219-233. DOI: 10.1111/jpy.13079.

Sauriau, P. G.; Dartois, M.; Becquet, V.; Aubert, F.; Huet, V.; Bréret, M.; Pante, E. 2021. Multiple genetic marker analysis challenges the introduction history of Ulva australis (Ulvales, Chlorophyta) on French coasts. J. Phycology. 56 455-467. DOI: 10.1002/gepi.21727.

Lee, H.; Kim, G.; Depuydt, S.; Shin, K.; Han, T.; Park, J. 2023. Metal Toxicity across Different Thallus Sections of the Green Macroalga, Ulva australis. Toxics. 11 548. DOI: 10.1016/j.jhazmat.2023.131039.

Kendel, M.; Wielgosz-Collin, G.; Bertrand, S.; Roussakis, C.; Bourgougnon, N.; Bedoux, G. 2015. Lipid composition, fatty acids and sterols in the seaweeds Ulva armoricana, and Solieria chordalis from Brittany (France): An analysis from nutritional, chemotaxonomic, and antiproliferative activity perspectives. Mar. Drugs. 13 5606-5628. DOI: 10.3390/md13095606.

Hardouin, K.; Bedoux, G.; Burlot, A. S.; Donnay-Moreno, C.; Bergé, J. P.; Nyvall-Collén, P.; Bourgougnon, N. 2016. Enzyme-assisted extraction (EAE) for the production of antiviral and antioxidant extracts from the green seaweed Ulva armoricana (Ulvales, Ulvophyceae). Algal Res. 16 233-239. DOI: 10.1016/j.foodchem.2016.05.047.

Lagourgue, L.; Gobin, S.; Brisset, M.; Vandenberghe, S.; Bonneville, C.; Jauffrais, T.; Payri, C. E. 2022. Ten new species of Ulva (Ulvophyceae, Chlorophyta) discovered in New Caledonia: Genetic and morphological diversity, and bloom potential. European J. Phycol. 57 458-478. DOI: 10.1080/09670262.2022.2027023.

Hu, M.; Zhao, S.; Liu, J.; Tong, Y.; Xia, Z.; Xia, J.; Zhang, J. 2022. The morphology, genetic diversity, and distribution of Ulva meridionalis (Ulvaceae, Chlorophyta) in Chinese Seas. J. Mar. Sci. Eng. 10 1873. DOI: 10.3390/jmse10121873.

Tran, L. A. T.; Vieira, C.; Steinhagen, S.; Maggs, C. A.; Hiraoka, M.; Shimada, S.; Leliaert, F. 2022. An appraisal of Ulva (Ulvophyceae, Chlorophyta) taxonomy. J. App. Phycol. 34 2689-2703. DOI: 10.1007/s10811-022-02815-x.

Costa, M. M.; Pio, L. B.; Bule, P.; Cardoso, V. A.; Duarte, M.; Alfaia, C. M.; Prates, J. A. 2022. Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases. Anim. Nutr. 9 184-192. DOI: 10.1016/j.aninu.2022.01.004.

Steinhagen, S.; Kramár, L.; Toth, G. B. 2023. The unheeded existence of the tubular greens: molecular analyses reveal the distribution of a new Ulva species (Ulvophyceae, Chlorophyta), Ulva capillata sp. nov. in the Atlantic-Baltic Sea transect. J. App. Phycol. 35 509-522. DOI: 10.1007/s10811-022-02886-w.

Han, H.; Zhao, S.; Song, X.; Wang, H. 2023. The overwintering capability of Ulva prolifera spores and gametes in the Yellow Sea, China. J. Ocean. 22 509-516. DOI: 10.1007/s11802-023-5260-y.

Jacobsen, M.; Bianchi, M.; Trigo, J. P.; Undeland, I.; Hallström, E.; Bryngelsson, S. 2023. Nutritional and toxicological characteristics of Saccharina latissima, Ulva fenestrata, Ulva intestinalis, and Ulva rigida: a review. Inter. J. Food Propert. 26 2349-2378. DOI: 10.1080/10942912.2023.2246677.

Farasat, N.; Sheidai, M.; Riahi, H.; Koohdar, F. 2022. Study of morphological, anatomical characteristics and metabolic assay of some Ulva species (sea lettuce) in the coastal waters of the Persian Gulf, Iran. Nova Biol. Rep. 9 222-235. DOI: 10.32687/0869-866X-2022-30-5-926-932.

Lagourgue, L.; Gobin, S.; Brisset, M.; Vandenberghe, S.; Bonneville, C.; Jauffrais, T.; Payri, C. E. 2022. Ten new species of Ulva (Ulvophyceae, Chlorophyta) discovered in New Caledonia: Genetic and morphological diversity, and bloom potential. J. Phycol. 57 458-478. DOI: 10.1080/09670262.2022.2027023.

Liu, W.; Liu, Q.; Zhao, J.; Wei, X.; Jiang, P. 2022. Comparative chloroplast genomes of Ulva prolifera and U. linza (Ulvophyceae) provide genetic resources for the development of interspecific markers. J. Oceanol. Limnol. 40 2372-2384. DOI: 10.3389/fgene.2022.1026919.

Kong, S.; Chen, Z.; Zhao, F.; Liu, Q.; Li, J. 2023. Cultivation of Ulva lactuca changes bacterial community structure and abundances of nitrogen cycling functional genes in an IMTA system. Res. 4 35. DOI: 10.1016/2023.739463.

Favot, E. J.; Holeton, C.; DeSellas, A. M.; Paterson, A. M. 2023. Cyanobacterial blooms in Ontario, Canada: Continued increase in reports through the 21st century. Lake and Reser. Management. 39 1-20. DOI: 10.1080/10402381.2022.2157781.

Steinhagen, S.; Hoffmann, S.; Pavia, H.; Toth, G. B. 2023. Molecular identification of the ubiquitous green algae Ulva reveals high biodiversity, crypticity, and invasive species in the Atlantic-Baltic Sea region. Algal Res. 103132. DOI: 10.1016/j.algal.2023.103132.

Masakiyo, Y.; Shimada, S. 2014. Species diversity of the genus Ulva (Ulvophyceae, Chlorophyta) in Japanese waters, with special reference to Ulva tepida Masakiyo et S. Shimada sp. nov. Bulletin of the Nat. Museum of Nature Sci. Botany. 40 1-13.

Melton III, J. T.; Lopez‐Bautista, J. M. 2021. Diversity of the green macroalgal genus Ulva (Ulvophyceae, Chlorophyta) from the east and gulf coast of the United States based on molecular data. J. Phycology. 57 551-568. DOI: 10.1127/pip/2021/0059.

Zhang, L.; Wang, G.; Liu, C.; Chi, S.; Liu, T. 2014. Development and utility of EST-SSR markers in Ulva prolifera of the South Yellow Sea. Oceanol. Acta. 33 105-113.

Zhang, J.; Kim, J. K.; Yarish, C.; He, P. 2016. The expansion of Ulva prolifera OF Müller macroalgal blooms in the Yellow Sea, PR China, through asexual reproduction. Maret. Pollut. Bull. 104 101-106. DOI: 10.1016/j.marpolbul.2016.01.056.

Dominguez, H.; Loret, E. P. 2019. Ulva lactuca, a source of troubles and potential riches. Mar. Drugs. 17 357. DOI: 10.3390/md17060357.

Van Alstyne, K. L.; Koellermeier, L.; Nelson, T. A. 2007. Spatial variation in dimethylsulfoniopropionate (DMSP) production in Ulva lactuca (Chlorophyta) from the Northeast Pacific. Mar. Biol. 150 1127-1135. DOI: 10.1007/s00227-006-0448-4.

Ponce, M.; Zuasti, E.; Anguís, V.; Fernández-Díaz, C. 2020. Effects of the sulfated polysaccharide ulvan from Ulva ohnoi on the modulation of the immune response in Senegalese sole (Solea senegalensis). Fish Shell. Immunol. 100 27-40. DOI: 10.1016/j.fsi.2020.02.054.


Full Text: PDF

DOI: 10.24815/jn.v24i1.33972

Refbacks

  • There are currently no refbacks.