The Effect Of Cellulase Content On Phytoestrogens Formation (Genistein) Materials On Fermented Soybean Waste Aspergillus niger

Vara Tassa Sutari, Nurliana Nurliana, Sugito Sugito, Darmawi Darmawi, Rinidar Rinidar

Abstract


The activity of the cellulase enzyme and the content of phytoestrogen (genistein) forming is determined during the fermentation of soybean waste with Aspergullus niger. The analysis is performed at fermentation incubation time of 0, 48, 96, 144 and 192 hours and treatment with addition of micro nutrient (V2) and without micro nutrient (V1). Data analysis on cellulase enzyme activity on phytoestrogen forming material is performed using T test. The average of cellulase content of fermented soybean waste increases from 0.032-0.139 IU / ml (V1) and 0.061-0.158 IU / ml (V2) after 48 hours into 0.238 IU / ml (V1) and 0.245 IU / ml (V2) after 192 hours The average of genestein levels in the fermented soybean waste increases as well from 0.101 - 0.573 mg / g (V1) and 0.114 - 0.587 mg / g (V2) after 48 hours and increase into 0.722 mg / g (V1) and 0.699 mg / g (V2) after 192 hours. Statistical analysis indicates that the treatment of V1 and V2 has an effect on the increase of cellulase enzyme content and genistein level (P <0.05). The correlation between enzyme activity of cellulase and genistein on soybean waste fermented with Aspergillus niger is significant (R2 = 0,949). The relationship between the enzyme performance and its formation material is running synergistic, cellulase enzyme activity and genistein level experience significant increase during the fermentation process.

Keywords


Soybean Waste; Aspergillus niger; Fermentation; Cellulase Enzyme and Genistein

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References


Balasarvana, T., John, D and Rathnan, R.K. 2013. Isolation, Screening Indetification and Optimized Produktion of Extracellular Cellulase from Bacillus subtilis using Cellulosic waste as Carbon Source. Jurnal of Microbiology, Biotechnology and Food Science. 2(6):2383-2386.

Chae, G.Y., Ha, B.J. 2011. The comparative evaluation of fermented and non-fermented soybean extract on anti-oxidantion and whitening. Toxicol Res. 27(40): 205209.

Chang, T.S. 2009. An update review of tyrosinase inhibitors. Int J Mol Sci. 10: 24402475.

Chiou, R.Y., Cheng, S.L, 2001. Isoflavon transformation during soybean koji preparation and subsequent miso fermentation suplemented with ethanol and NaCl. J Agric Food Chem. 49(8): 3656-3660.

Desi, A. 2012. Aktivitas enzim selullase isolat SGS 2609 BBP4B_KP menggunakan substrat limbah pengolahan rumput laut yang dipretreatment dengan asam. Skripsi F.T Universitas Indonesia. Depok.

Dhaubhadel, S. 2011. Regulation of isoflavonoid biosynthesis in soybean seeds. Canada: Southern crop protection and food research center; p.243-258.

Fani, C. Nurliana dan Razali. 2013. Efek pemberian pakan yang mengandung ampas kedelai terfermentasi Aspergillus niger terhadap ketebalan kerabang telur ayam kampung (Gallus domesticus). Jurnal Medika Veteriner vol.7(64-66).

Garlok, T. 2000. The effect of various acidic solutions on the concentration genistein in tempeh. Tesis. The graduate college University of Wisconsin-Stout Menomonie.

Glover, A. Assinder S.J. 2006. Acute exposure of adult male rats to dietary phytoestrogen reduces fecundity and alters epididymal streroid hormon receptor expression. J. Endocrinol. 189:565-573.

Huynh, N.T., Camp, J.V., Smagghe, G., and Raes, K. 2015. Improved release and metabolism of flavonoids by steered fermentation processes: A review. Int J Mol Sci. 15:19369-19388.

Isabella, D.C.C. 2008. Quantitation of genistein and genistin in soy dry extracts by UV-Visible spektrophotometric method. Vol. 31, no. 8. Departemento de produtos farmaceuticos, faculdade de farmacia, Univeridade federal de minas gerais, Av. Pres Antonio Carlos. Brasil

Jefferson, W.N., E. Padilla-Banks, G. Clark, and R.R. Newbold. 2002. Assessing estrogenic activity of phytochemicals using transcriptional activation and immature mouse uterotrophic responses. Journal of Chromatography, 777(1-2):179-189.

Kalsum, U. O, and Sjofjan (2008). Pengaruh waktu inkubasi campuran ampas tahu dan ongkok yang difermentasi dengan Neurospora sithophila terhadap kandungan zat makanan. Seminar Nasional Teknologi Peternakan dan Veteriner. Universitas Islam Malang. Malang .

Lingga, R. 2010. Uji Nematisidal Jamur Endofit Tanaman Padi (Oryza sativa L.) terhadap Nematoda Puru Akar (Meloidogyne spp). Skripsi. Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Sumatera Utara. Medan.

Maier, R.M., I.L. Pepper, dan C.P. Gerba. 2000. Environmental Microbiology. Academic Press, London.

Maryandini, A., Widosari, W., Maranatha, B., Sunarti and Rahmania, T.C. 2009. Isolasi Bakteri Selulotik dan karakterisasi enzimnya. Makara Sains 13:33-38.

Muis, H., I. Martaguri, dan Mirnawati. 2009. Teknologi Bioproses Ampas Kedelai untuk Meningkatkan Daya Gunanya. Laporan Peneliian Fundamental DIKTI, Universitas Andalas, Padang.

Murray M.J., Meyer W.R., Lessey B.A., Oi R.H., DeWire R.E., Fritz M.A. 2003. Soy protein isolate with isoflavones does not prevent estradiol-induced endometrial hyperplasia in postmenopausal women: a pilot trial. Menopause. 10(5):456-464.

Nurliana, Razali, dan C. Fani. 2013. Efek Pemberian pakan yang mengandung ampas kedelai terfermentasi Aspergillus niger terhadap ketebalan kerabang telur ayam kampung (Gallus domesticus). Jurnal Medika Veterinaria. 7(2):65-66.

Pandit, N.T., Patravele, V.B. 2011. Design and optimization of a novel method for extraction of genistein. Indian J Pharma Scie. 73(20: 184-192.

Pawiroharsono, S. 2001. Prospek dan Manfaat Isoflavon untuk Kesehatan. Direktorat Teknologi Bioindustri, Badan Pengkajian dan Penerapan Teknologi, Yogyakarta.

Purwadaksi, 2007. Membuat Tempe dan Tahu. Agromedia Pustaka. Jakarta.

Putri, Y., C.N. Thasmi., M. Adam, dan Nurliana. 2013. Efek pemberian ampas kedelai nonfermentasi dan yang difermentasi Aspergillus niger terhadap jumlah folikel telur ayam kampong (Gallus domesticus). Jurnal Medika Veterinaria. 7(2):77-78.

Safriani, 2013. Kajian Kondisi Fermentasi pada Produksi Selulase dari Limbah Kelapa Sawit (Tandan Kosong dan Sabut) oleh Neurospora sitophila. Skripsi. Jurusan Teknologi Industri Pertanian, Fakultas Teknologi Pertanian, Institut Pertanian Bogor.

Sinurat, A.P., T. Purwadaria, T. Pasaribu, J. Darma, I.A.K. Bintang dan M.H. Togatorop. 2001. Pemanfaatan lumpur sawit untuk ransum unggas: 4. Penggunaan produk fermentasi lumpur sawit sebelum dan setelah dikeringkan dalam ransum ayam kampong sedang tumbuh. JITV 6: 213-219.

Suprihatin, 2010 Teknologi Fermentasi. Surabaya: UNESA pers.

Tagliaferri. M., I. Cohen., D. Tripathy, 2007. Kanker payudara. Jakarta: PT. Indeks.

Teekachunhatean., S., Hanprasertpong, N., and Teekachunhatean, T. 2013. Factors affecting isoflavone content in soybeanseeds grown in Thailand. Int J Agronomy. 163573: 111.

Ul-Haq, I., Javed. M. M., Khan. T. S. and Siddiq, Z. 2005. Cotton saccharifying activity of cellulases produced by co-culture of Aspergillus niger and Trichoderma viridae. Research Journal of Agriculture and Biological Sciences, 1(3): 241- 245.

Winarsi, H. 2005. Isolavon Bernbagai Sumber, Sifat dan Manfaatnya Pada Penyakit Degeneratif. Gadjah Mada University Press, Yogyakarta.

Wood, 1998. Organization Behavior: A Global Perspective. New York: John Wiley and Sons Andbulia, Ltd.

Yuan, D., Chen, Y., Bai, X., Pan, Y., and Kano, Y. 2006. TLC and HPLC analysis of soy isoflavones in semen sojae praeparatum. Asia J Tradisional Med. 1: 3-4.

Yunindarwati, E., E.U. Ulfa., E. Puspitasari and M.A. Hidayat. 2015. Pengaruh Fermentasi Aspergillus oryzae terhadap Kadar Genistein Kedelai. Fakultas Farmasi, Universitas Jember. Jember.




DOI: https://doi.org/10.21157/ijtvbr.v3i1.11362

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