Dhesy Kartikasari, Sri Mulyati, Suzanita Utama, Pudji srianto, Widjiati Widjiati, Hani Plumeriastuti


This study aims to evaluate the expression of BAX, BCL-2, and BAX/BCL-2 ratio in maturation media of cow oocytes which supplemented with Urea in vitro because BAX and BCL-2 are the main regulators of apoptosis. A total of 263 oocytes from follicle aspirations originating from ovaries taken from slaughterhouses and were saturated with 3 addition of urea which was divided into three groups. The control group (P0) was control group without the addition of urea, P1 group was added with urea 20 mg/dL, while P2 group was added with urea 40 mg/dL. The results of in vitro oocyte maturation were continued with identification using immunocytochemical staining with the addition of BAX and BCL-2 antibodies. Positive results showed a brownish color on the oocyte and its cumulus. The results of this study indicated that there were significant differences (P<0.05) in BAX and BCL-2 expression, although both curves were equally increase. The increase in BCL-2 was more significant than BAX, while the BAX BCL-2 ratio did not show a significant difference (P>0.05) in whichthe curve of BAX/BCL-2 ratio was decreased. It can be concluded that the addition of urea does not affect the level of apoptosis.


apoptosis, BAX/BCL-2 ratio, oocyte, urea

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Allaire, A.D., Ballenger, K.A., Wells, S.R, McMohan, M.J, and Lessey, B.A. (2000), Placental Apoptosis in Preeclampsia, Obstetrics & gynecology, vol 96, No 2, University of North Carolina at chapel hill, Chapel Hill, North Carolina.

Bazet, M.A., K.S. Huque, N. R. Sarker., M.M. Hossain., dan M.N. Islam. 2010. Evaluation f Milk Urea Nitrogen of Dairy Cows Reared Under Different Feed Bases in the Different Seasons. J. Sci. Foundation. 8 (1&2): 97-110.

Dhali A., D. P. Mishra, R. K. Mehla, and S. K. Sirohi. 2006. Usefulness of milk urea concentration to monitor the herd reproductive performance in crossbred karan-fries cow. J. Anim. Sci. 19: 26-30.

Fink SL, Cookson BT. Apoptosis, pyroptosis, and necrosis: mechanistic description of dead and dying eukaryotic cells. Infect Immun. 2005;73(4):1907-16

Firmiaty, S., G. Ciptadi, S. Wahjuningsih dan Suyadi. 2014. Effect of Insulin Transferrin Selenium (ITS) on Oocyte Maturation In Vitro in Indonesian Goats. Jurnal of Biology. Agriculture and Healthcare 4 (7):113-117.

Galluzzi L1, Vitale I, Abrams JM, Alnemri ES, Baehrecke EH, Blagosklonny MV, Dawson TM, Dawson VL, El-Deiry WS, Fulda S, Gottlieb E, Green DR, Hengartner MO, Kepp O, Knight RA, Kumar S, Lipton SA, Lu X, Madeo F, Malorni W, Mehlen P, Nuñez G, Peter ME, Piacentini M, Rubinsztein DC, Shi Y, Simon HU, Vandenabeele P, White E, Yuan J, Zhivotovsky B, Melino G, Kroemer G., 2012. Molecular definitions of cell death subroutines: recommendations of the Nomenclature Committee on Cell Death 2012. 19(1):107-20.

Garuti G, Grossi F, Centinaio G, Sita G, Nalli G, Luerti M. Accuracy of hysteroscopy in the diagnosis of endometrial cancer and hyperplasia: a systematic quantitative review. JAMA 2002;288(13):1610-21.

Ghobrial IM, Witzig TE, Adjei AA. 2005. Targeting apoptosis pathways in cancer therapy. CA Cancer J Clin. 55:178-94.

Gross, McDonnel and Korsmeyer BCL-2 Family Members and The Mitochondria in Apoptosis. Genes and Development. 1999:13 1899-1911

Hoffman G.E., W.W. Le, and L.V. Sita. 2008. The Importance of Titrating Antibodies for Immunocytochemical Methods. In Current Protocols in Neuroscience. Wiley Interscience. John Wiley and Sons. 1-26.

Hoi, P. M., Li, S., Vong, C.T., Tseng, H.H.L., Kwan, Y.W., Lee, S.M-Y., 2015. Recent Advances in Structure Advances in Structure-Based Drug Design and Virtual Screening Of Vegfr Tyrosine Kinase Inhibitors. Methods, 71, Pp. 85-91

Kumar V, Abbas AK, Fausto N. Robbins and Cotran. 2005. Pathologic basis and disease. Edisi ke-7. Philadelpia: Elsevier Sounders.

Kusriningrum, R.S. 2011. Buku Ajar Perancangan Percobaan. Cetakan ketiga. Fakultas Kedokteran Hewan Universitas Airlangga. Penerbit Dani Abadi. Surabaya.

McGavin, M.D., Lopez, A. and Zachary, J.F., 2007. Pathologic Basis of Veterinary Disease.

Putri K.Y., P. Srianto, T.D. Lestari, S. Utama, Wurlina, I. Mustofa. 2018. Reproductive Efficiency and Serum Progesteron Concentration on Dairy Catlle Based on Blood Urea Nitrogen (BUN) Concentrations. Iraqi Journal of Veterinary Sciences, Vol. 32, No. 2, 2018 (143-148).

Reed JC. 1994. Bcl-2 and the regulation of programmed cell death. J Cell Biol. 124:1-6.

Ruiz-vela, A, Opferman, J. T., Cheng, E. H., & Korsmeyer, S. J., (2005), scientific report, Molecular Biology, 6(4), 2-8. doi:10.1038/sj.embor,7400375.

Shiozawa T, Konishi I. 2006. Early endometrial carcinoma: clinicopathology, hormonal aspects, molecular genetics, diagnosis, and treatment. Int J Clin Oncol. 11:13-21.

Sinclair. 2008. Komposisi Asam amino dan asam lemak pada cairan folikel sebagai prediktor perkembangan fertilisasi in vitro. Reprod biomed. On line 16,859-868.

Tamm I, Schriever F, Dorken B. 2001. Apoptosis: implications of basic research for clinical oncology. Lancet Oncol. 2:33-42.

Vaskivuo TE, Stenback F, Tapanaenin JS. Apoptosis and apoptosis-related factors Bcl-2, Bax, tumor necrosis factor-alpha, and NF-kappaB in human endometrial hyperplasia and carcinoma. Cancer 2002 1;95(7):1463-71.

Wattimena, J. 2011. Pematangan Oosit Domba Secara In Vitro dalam Berbagai Jenis Serum. Agriminal 1 (1): 22-27.

Widjiati, D. Wulansari, Wurlina, dan N.M.R Widjaja. 2011. Identifikasi Growth Differentiation Factor (GDF-9) Dari Maturasi In Vitro Oosit Sapi Dengan Teknik Imunositokimia. Jurnal Kedokteran Hewan. 5(2): 59-62.


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