Koko Wisnu Prihatin, Sucik Maylinda, Luqman Hakim


The aim of this study was to determine the diversity of the sex-determining region Y (SRY) gene in Madura cattle bulls that had specifically selected for the production of frozen semen. DNA was isolated from the whole blood derived from 5 Madura cattle bulls and the SRY gene amplification carried out using polymerase chain reaction (PCR) with SRY-4 as a primer followed by DNA sequencing. Results revealed the proximity of the Madura cattle SRY genes to the Bos indicus SRY genes, furthermore this study also proved the existence of variation in Madura cattle SRY gene caused by mutation and deletion of nucleotides. It was concluded that the variations in Madura cattle SRY genes still persist even in the specifically selected populations with similar phenotype.


Madura cattle; SRY variation; Sequence analysis

Full Text:



Kikkawa Y., T. Takada., Sutopo, K. Nomura, T. Namikawa, H. Yonekawa and T. Amano. 2003. Phylogenies using mtDNA and SRY provide evidence for male-mediated introgression in Asian domestic cattle. Animal Genetics 34: 96–101.

Li Y., M. Zheng, and Y.C. Lau. 2014. The sex-determining factors SRY and SOX9 regulate similar target genes and promote testis cord formation during testicular differentiation. Cell Reports 8: 723–733.

Liu W.S. and F.A. Ponce deLeón. 2007. Mapping of the Bovine Y Chromosome. Electronic Journal of Biology 3(1): 5-12.

Lu, L., F. Hu, and S. Ge. 2013. Investigation on Azoospermia Factor (AZF) Microdeletion and Sex-determining Region Y (SRY) of the Y Chromosome in Male Infertility. Journal of Reproduction and Contraception 24(2):88–94.

Mburu, D. and O. Hanotte. 2005. A practical approach to microsatellite genotyping with special reference to livestock population genetics. ILRI Biodiversity project. ILRI, Kenya. Page 8.

Mohamad K., M. Olsson, H.T.A. van Tol, S. Mikko, B.H. Vlamings, G. Andersson, H. Rodriguez-Martines, B. Purwantara, R.W. Paling, B. Colenbrander and J.A. Lenstra. 2009. On the Origin of Indonesian Cattle. PLoS ONE 4(5): 10.1371

Mukherjee, A., G. Dass, J.M. G, M. Gohain, B. Brahma, T.K. Datta, and S. De. 2013. Absolute copy number differences of Y chromosomal genes between crossbred (Bos taurus × Bos indicus) and Indicine bulls. J. Anim. Sci. Biotechnol. 4(1):15.

Nijman I.J., M. Otsen, E.L. Verkaar, C. deRuijter, E. Hanekamp, J.W. Ochieng, S. Shamsaad, J.E. Rege, O. Hanotte, M.W. Barwegen, T. Susilawati and J.A. Lenstra. 2003. Hybridization of Banteng (bos Javanicus) and Zebu (Bos Indicus) Revealed by Mitocondrial DNA, Satelite DNA, AFLP and Microsatellite. Heredity 90(1):10-16.

Nijman, I.J., D.C.J. van Boxtel, L.M. van Cann, Y. Marnoch, E. Cuppen, and J.A. Lenstra. 2008. Phylogeny of Y-chromosomes from bovine species. Cladistics 24 (5): 723-726.

Payne, W.J.A and D.H.L. Rollinson, 1976. Madura cattle, Z Tierzuch Zu chtsbiol 93: 89–100.

Sambrook, J., E.F. Fritsch, and T. Maniatis, 1989. Molecular cloning : a laboratory manual. Cold Spring Harbor Laboratory Press. New York. USA.

Syed-Shabthar, S.M.F., M.K.A. Rosli, N.A.A. Mohd-Zin, S.M.N. Romaino, Z.A. Fazly-Ann, M.C. O. Abas-Maszni, R. Zainuddin, S. Yaakop and B.M. Md-Jain. 2013. The molecular phylogenetic signature of Bali cattle revealed by maternal and paternal markers. Mol Biol Rep 40(8):5165-76.

Verkaar, E.L.C., H. Vervaecke, C. Roden, L. Romero-Mendoza, M.W. Barwegen, T. Susilawati, I.J. Nijman and J.A. Lenstra. 2003. Paternally inherited markers in bovine hybrid populations. Heredity 91, 565–569.


Article Metrics

Abstract view : 0 times
PDF - 0 times


  • There are currently no refbacks.

Copyright (c) 2020 by author and J. Kedokt. Hewan

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indexed by:


p-ISSN: 1978-225X e-ISSN: 2502-5600 Copyright© 2007-2021