24. Antimicrobial Activity of Ant Plant (Myrmecodia sp.) Water Fraction to Escherichia coli and Salmonella sp. Growth

Roslizawaty Roslizawaty; Robbi Ghani; Maryulia Dewi; Darniati Darniati; T. Armanyah TR; Ginta Riady; Nuzul Asmilia

doi:10.21157/j.med.vet..v13i2.3653

24. Antimicrobial Activity of Ant Plant (Myrmecodia sp.) Water Fraction to Escherichia coli and Salmonella sp. Growth

Roslizawaty Roslizawaty, Robbi Ghani, Maryulia Dewi, Darniati Darniati, T. Armanyah TR, Ginta Riady, Nuzul Asmilia

Abstract

The aim of this study was to determine antimicrobial activity of sarang semut (Myrmecodia sp.) to Escherichia coli and Salmonella sp. growth using Kirby Bauer methode. This study used Completely Randomized Design one way pattern. Sample used was water fraction of sarang semut with concentration of 25% (P2), 50% (P3), and 75% (P4). Positive control was amoxycillin and negative control was Aquadest. Data were analyzed by Analysis of Varians (ANOVA). Average clear zone formed on Escherichia coli of P0, P1, P2, P3, and P4 were 0; 18,76; 5,96; 6,4; and 7,8 mm, respectively. Average clear zone formed on Salmonella sp. of P0, P1, P2, P3, and P4 were 0; 33,63; 7,73; 10,5; and 11,56 mm, respectively. ANOVA analysis showed there was correlation between extract concentration and inhibition zone on each culture of Escherichia coli and Salmonella sp. T test on P2, P3, and P4 showed that this extract had better antimicrobial effect on Salmonella sp. compared to Escherichia coli. In conclusion, water fraction of Myrmecodia sp. had capability to inhibit Escherichia coli and Salmonella sp. growth.

Keywords

water fraction; inhibition zone; ant plant (Myrmecodia sp.); Escherichia coli; Salmonella sp.

Full Text:

PDF

References

Achmad, H., M.H. Chandha, S. Ramadhany, H. Handayani, and R. Samad. 2014. The role of sarang semut (Myrmecodia pendans) flavonoid’s fraction in proliferation and angiogenesis inhibition of human tongue squamous cell carcinoma. J. Biology, Agriculture and Healthcare. 4(21): 65-69.

Andriani. 2005. Escherichia coli 0157 H:7 sebagai penyebab Penyakit Zoonosis. Prosiding Lokakarya Nasional Penyakit Zoonosis. Bogor. 173-177.

Cumming, P.L., F. Sorvillo, and T. Kuo. 2012. The Burden of Salmonellosis in the United States. Mahmoud, B. S. M. (ed). Salmonella – a Dangerous Foodborne Pathogen. InTech Open Access Publisher. Kroasia.

Davies, R. 2010. Salmonellosis. Manual of diagnostic tests and vaccines

for terrestrial animals 2015. OIE Terrestrial Manual. Paris.

Frengki, Roslizawaty, dan D. Pertiwi. 2014. Uji toksisitas ekstrak etanol sarang semut lokal Aceh (Mymercodia sp.) dengan metode BSLT terhadap larva udang Artemia salina leach. J. Medika Veterinaria. 7(1): 60-62.

Hermawati, R. dan D. Arumsari. 2014. Khasiat Ajaib Sarang Semut Berantas Berbagai Penyakit. Penerbit Padi, Jakarta.

Jasmine, R., B.N. Selvakumar, and P. Daisy. 2011. Investigating the mechanism of action of terpenoids and the effect of interfering substances on an indian

medicinal plant extract demonstrating antibacterial activity. International Journal of Pharmaceutical Studies and Research. 2(2): 19-24.

Kohanksi, M.A., D.J. Dwyer, and J.J. Collins. 2010. How Antibiotics Kill Bacteria: From Target Networks. Nature Reviews Microbiology. 8(12): 423-435.

Morales, G., P. Sierra, A. Mancilla, A. Paredes, L.A Loyola, O. Galardo, and J. Borquez. 2003. Secondary metabolites from four medicinal plants from northern chile: antimicrobial activity and biotoxicity against Artemia salina. J. Chil. Chem. Soc (online version). 48(2).

Kristina, D. 2008. Efek Antiinflamasi Ekstrak Etanol Umbi Sarang Semut (Myrmecodia pendens merr. & perry) Pada Tikus Putih (Rattus norvegicus l.). Skripsi. Jurusan Biologi, Universtas Sebelas Maret, Surakarta.

Pratisto, S.D. 2006. Sarang Semut Multi Khasiat. Majalah Gatra, Jakarta

Roslizawaty, N.Y. Ramadani, Fakhrurrazi dan Herrialfian. 2013. Aktivitas antibakterial ekstrak etanol dan rebusan sarang semut (Myrmecodia sp.) terhadap bakteri Escherichia coli. J. Medika Veterinaria. 7(2): 91-94.

Todar, K. 2007. Pathogenic Escherichia coli. Online Textbook of Bacteriology. University of Wisconsin–Madison Department of Bacteriology, United States of America.

Zhu, Y.Z., B. K-H Tan, B. Bay, and C.H. Liu. 2007. Natural Products, Essential Resources for Human Survival. World Scientific Publishing, England.

DOI: https://doi.org/10.21157/j.med.vet..v13i2.3653