Analysis of Total Anthocyanin Levels in Hibiscus (Hibiscus rosa-sinensis L.) Preparations by Differential pH Method Using UV-Vis Spectrophotometry

Elsa Suryani*, Laili Nailul Muna

Abstract


Red hibiscus is a plant that contains anthocyanins and flavonoids which act as natural dyes and preservatives. Anthocyanins are attractive coloring substances and are not harmful to health, therefore they are widely used in the food and pharmaceutical industries. The maximum allowable daily intake of anthocyanins is 0.25 mg/kg body weight. Excessive intake of anthocyanins can cause poisoning. This study aims to determine the total anthocyanin content in hibiscus extract. The method used to analyze anthocyanin levels is the differential pH method using UV-Vis spectrophotometry. The main advantage of this method is that it provides a simple way to measure very small amounts of substances. The results of the research explained that the qualitative analysis showed that the sample was positive for containing anthocyanins because the acid reagent HCl remained red, this was due to the formation of flavilium. The sample with the base reagent (NaOH) changes color to green due to the formation of a carbinol pseudobase structure. Quantitative analysis of anthocyanins showed that in 10.8876 grams of extract there was an anthocyanin content of 72.4731 mg/L at a dilution of 10 ml. The high level of total anthocyanin in the thick hibiscus extract preparation can be influenced by several things such as the temperature during the drying process, the length of maceration time, the concentration of the solvent used, and the high intensity of light


Keywords


Anthocyanin; Hibiscus Extract; Differential pH

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Afandi, R., & Purwanto, A. 2018. Spektofotometer cahaya tampak sederhana untuk menentukan panjang gelombang serapan maksimum larutan Fe(SCN)3 dan CuSO4 simple visible light spectroscopy to determine the maximum absorbance wavelength. Journal Spektrofotometer Cahaya Tampak, 2(4):116–130.

Agustin, D. & Ismiyati, I. 2015. Pengaruh konsentrasi pelarut pada proses ekstraksi antosianin dari bunga kembang sepatu. Jurnal Konversi, 4(2):9-16. https://doi.org/10.24853/konversi.4.2.9-16.

Armanzah, R.S. & Hedrawati, T.Y. 2016. Pengaruh Waktu Maserasi Zat Antosianin Sebagai Pewarna Alami dari Ubi Jalar Ungu (Ipomoea batatas L. Poir). Seminar Nasional Sains dan Teknologi, 19(2):1–10. jurnal.umj.ac.id/index.php/semnastek%0Ap.

Cahyaningsih, P.E.S.K.Y.E., Winariyanthi, & Yuni, N.L.P. 2017. Skrining Fitokimia dan Analisis Kromatografi Lapis Tipis Ekstrak Tanaman Patikan Kebo (Euphorbia hirta L.). Jurnal Ilmiah Medicamento, 3(2):1–10.

Charmongkolpradit, S., Somboon, T., Phatchana, R., Sang-Aroon, W., & Tanwanichkul, B. 2021. Influence of drying temperature on anthocyanin and moisture contents in purple waxy corn kernel using a tunnel dryer. Case Studies in Thermal Engineering, 25:100886. https://doi.org/10.1016/j.csite.2021.100886.

Gustriani, N., Novitriani, K., & Mardiana, U. 2016. Penentuan Trayek pH Ekstrak Kubis Ungu (Brassica oleracea L) Sebagai Indikator Asam Basa Dengan Variasi Konsentrasi Pelarut Etanol. Jurnal Kesehatan Bakti Tunas Husada: Jurnal Ilmu-Ilmu Keperawatan, Analis Kesehatan Dan Farmasi, 16(1):94-99. https://doi.org/10.36465/jkbth.v16i1.171.

Herlina, H., Jannah, S., Mulyani, E., & Sembiring, M. 2023. Analisa Antosianin Pada Minuman Olahan Bunga Telang (Clitoria Ternatea L) Dengan Metode pH Differensial. Parapemikir :Jurnal Ilmiah Farmasi, 12(2):217-224. https://doi.org/10.30591/pjif.v12i2.5138.

Herlina lina, H., Mulyani, E., & Anlika, R. 2023. Analisa Antosianin Pada Sediaan Minuman Effervescent Sari Bunga Telang (Clitoria ternatea) Dan Jeruk Rimau Gerga Lebong (Citrus nobilis sp.) Menggunakan Metode pH Diferensial. Jurnal Insan Farmasi Indonesia, 6(2):211–221. https://doi.org/10.36387/jifi.v6i2.1653.

Ifadah, R.A., Wiratara, P.R.W., & Afgani, C.A. 2022. Ulasan Ilmiah : Antosianin dan Manfaatnya untuk Kesehatan. Jurnal Teknologi Pengolahan Pertanian, 3(2):11–21. https://doi.org/10.35308/jtpp.v3i2.4450.

Irawan, A. 2019. Kalibrasi Spektrofotometer Sebagai Penjaminan Mutu Hasil Pengukuran dalam Kegiatan Penelitian dan Pengujian. Indonesian Journal of Laboratory, 1(2): 1-7. https://doi.org/10.22146/ijl.v1i2.44750.

Jamei, R., & Babaloo, F. 2017. Stability of Blueberry (Cornus mas–Yulyush) Anthocyanin Pigment Under pH and Co-pigment Treatments. International Journal of Food Properties, 20(9):2128–2133. https://doi.org/10.1080/10942912.2016.1233116.

Lestari, T.S., & Hamzah, B. 2022. Analisis Kadar Senyawa Flavonoid Ekstrak Etanol Buah Pare (Momordica charantia L.). Media Eksakta, 18(2):96–101. https://doi.org/10.22487/me.v18i2.1505.

Meganingtyas, W., & Alauhdin, M. 2021. Ekstraksi Antosianin dari Kulit Buah Naga (Hylocereus costaricensis) dan Pemanfaatannya sebagai Indikator Alami Titrasi Asam-Basa. AgriTECH, 41(3):, 278. https://doi.org/10.22146/agritech.52197.

Navas, M.J., Jiménez-Moreno, A.M., Bueno, J.M., Sáez-Plaza, P., & Asuero, A.G. 2012. Analysis and Antioxidant Capacity of Anthocyanin Pigments. Part IV: Extraction of Anthocyanins. Critical Reviews in Analytical Chemistry, 42(4):, 313–342. https://doi.org/10.1080/10408347.2012.680343.

Ooe, E., Ogawa, K., Horiuchi, T., Tada, H., Murase, H., Tsuruma, K., Shimazawa, M., & Hara, H. 2016. Analysis and characterization of anthocyanins and carotenoids in Japanese blue tomato. Bioscience, Biotechnology and Biochemistry, 80(2):, 341–349. https://doi.org/10.1080/09168451.2015.1091715.

Pelletier, V., Caron, E., Caron, J., Gallichand, J., & Vanderleest, C. 2015. Impact of Irrigation Thresholds on Total Anthocyanin Content in Cranberries. Communications in Soil Science and Plant Analysis, 46(16):, 2095–2099. https://doi.org/10.1080/00103624.2015.1069314.

Pratiwi, S.W., & Priyani, A.A. 2019. Pengaruh Pelarut dalam Berbagai pH pada Penentuan Kadar Total Antosianin dari Ubi Jalar Ungu dengan Metode pH Diferensial Spektrofotometri. EduChemia (Jurnal Kimia Dan Pendidikan), 4(1):, 89. https://doi.org/10.30870/educhemia.v4i1.4080.

Priska, M., Peni, N., Carvallo, L., & Ngapa, Y.D. 2018. Antosianin dan Pemanfaatannya. Cakra Kimia (Indonesian E-Journal of Applied Chemistry), 6(2):, 79–97.

Purwaniati, P., Arif, A.R., & Yuliantini, A. 2020. Analisis Kadar Antosianin Total Pada Sediaan Bunga Telang (Clitoria ternatea) Denga Metode pH Diferensial Menggunakan Spektrofometri Visible. Jurnal Farmagazine, 7(1):, 18. https://doi.org/10.47653/farm.v7i1.157.

Puspitasari, A.D., & Prayogo, L.S. 2017. Perbandingan Metode Ekstraksi Maserasi dan Sokletasi Terhadap Kadar Fenolik Total Ekstrak Etanol Daun Kersen (Muntingia calabura). Jurnal Ilmiah Cendekia Eksakta, 1(2):1–8.

Roy, S., & Rhim, J.W. 2021. Anthocyanin Food Colorant and Its Application in pH-Responsive Color Change Indicator Films. Critical Reviews in Food Science and Nutrition, 61(14):, 2297–2325. https://doi.org/10.1080/10408398.2020.1776211.

Salamah, N., & Widyasari, E. 2015. Aktivitas Antioksidan Ekstrak Metanol Daun Kelengkeng (Euphoria longan (L) Steud.) Dengan Metode Penangkapan Radikal 2,2’-Difenil-1-Pikrilhidrazil. Pharmaciana, 5(1):25–34. https://doi.org/10.12928/pharmaciana.v5i1.2283.

Sampebarra, A.L. 2018. Karakteristik Zat Warna Antosianin Dari Biji Kakao Non Fermentasi Sebagai Sumber Zat Warna Alam Characterization of Antosianin Source of Natural Dyes From Unfermented Cocoa Beans As A Source of Natural Dyes. Jurnal Industri Hasil Perkebunan, 13(1):63–70.

Sangadji, I., Rijal, M., & Kusuma, Y.A. 2017. Kandungan Antosianin Di Dalam Mahkota Bunga Beberapa Tanaman Hias. Biosel: Biology Science and Education, 6(2):118. https://doi.org/10.33477/bs.v6i2.163.

Wijaya, H., Novitasari, & Jubaidah, S. 2018. Perbandingan Metode Ekstraksi Terhadap Rendemen Ekstrak Daun Rambui Laut (Sonneratia caseolaris L. Engl). Jurnal Ilmiah Manuntung, 4(1):79–83.

Zahroh, F., & Agustini, R. 2021. Penentuan Kandungan Total Antosianin Yeast Beras Hitam (Oryza sativa L. Indica) Menggunakan Metode pH Differensial. Unesa Journal of Chemistry, 10(2):200–208. https://doi.org/10.26740/ujc.v10n2.p200-208.




DOI: https://doi.org/10.24815/jipi.v8i3.40575

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