A New Properties of Bajakah Tampala Wood (Spatholobus littoralis Hassk) Ethanol Extract Against α-Amylase Inhibition as Candidate Antidiabetic and Its Fortification in Kapok Honey

Rista Anggriani, Noor Harini, Afifa Husna, Sukardi Sukardi, Maureen Danysha NS

Abstract


Bajakah Tampala extracted with ethanol was indicated to contain phenolic and flavonoids. So far, the ethanol extract of Bajakah Tampala wood has only been analyzed for its antioxidant activity. Whereas flavonoids not only act as antioxidants but also play a role in inhibiting -amylase which is related to antidiabetic properties. The ethanol extract of Bajakah Tampala wood has never been used as a fortificant in honey. The purpose of this study was to determine the levels of phenols, flavonoids, antioxidant activity, and inhibitory activity of α-amylase from the ethanol extract of Bajakah Tampala Wood (EEBTW) at various solvent concentrations. In addition, to investigate the effect of adding EEBTW to Kapok honey on its bioactivity. This study was conducted using Post Test Only Group Design that was applied to 1% fortification of the three extracts in Kapok honey. Extraction used three concentrations of ethanol (70%, 80%, and 90%). Parameters tested in the study were total phenol, total flavonoid, antioxidant activity, and % inhibition of α-amylase. The result showed the higher the ethanol concentration, the lower the total phenolic, flavonoid, % DPPH scavenging activity, and α-amylase inhibition. The addition of 1% EEBKT has synergism with brown honey in increasing phenol, antioxidant activity, and % inhibition of α-amylase. EEBTW has the potential as an antidiabetic candidate and Kapok honey with EEBTW has the potential as functional honey

Keywords


Antidiabetic; bajakah tampala wood; functional honey; inhibition of α-amylase

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References


Amiani, W., Fahrizal, M. R., & Aprelea, R. N. 2022. Kandungan Metabolit Sekunder Dan Aktivitas Tanaman Bajakah Sebagai Agen Antioksidan. Jurnal Health Science, 3(4), 516–522.

Anggriani, R., Sukardi, S., Winarsih, S., & Adinka, E. 2022. TOTAL FENOL , TOTAL FLAVONOID , AKTIVITAS ANTIOKSIDAN, DAN PENAMBAHAN SARI MENGKUDU. Jurnal Teknologi Pangan Dan Gizi, 21(1), 55–62.

Arifin, B., & Ibrahim, S. 2018. Struktur, Bioaktivitas Dan Antioksidan Flavonoid. Jurnal Zarah, 6(1), 21–29. https://doi.org/10.31629/zarah.v6i1.313

Arifin, Y. F., Hamidah, S., & Hatta, G. M. 2021. Comparison of the Flavonoid Contents of Bajakah Plants from Tropical Forest in Kalimantan, Indonesia. Journal of Hunan University Natural(Natural Sciences), 48(8), 22–26.

Asfar, A. M. I. A., & Yasser, M. 2018. Analisis Kualitatif Fitikomia Kandungan Flavonoid Ekstrak Kayu Sepang (Caesalpinia Sappan L.) dari Ekstraksi Metode Ultrasonic Assisted Solvent Extraction. Chemica: Jurnal Ilmiah Kimia Dan Pendidikan Kimia, 19(2), 143. https://doi.org/10.35580/chemica.v19i2.12772

Ayuchecaria, N., Saputera, M. M. A., & Niah, R. 2020. PENETAPAN KADAR FENOLIK TOTAL EKSTRAK BATANG BAJAKAH TAMPALA (Spatholobus littoralis Hassk.) MENGGUNAKAN SPEKTROFOTOMETRI UV-VISIBLE. Jurnal Insan Farmasi Indonesia, 3(1), 132–141. https://doi.org/10.36387/jifi.v3i1.478

Azzahra, A., Farhani, N., Syahfitri, W., & Pasaribu, S. F. 2022. Potensi Kandungan Flavonoid Dalam Kayu Bajakah Sebagai Antidiabetes. Jurnal Pendidikan Tambusai, 6(2), 14345–14350.

Brahmachari, G. 2011. Bio-flavonoids with promising anti- diabetic potentials : A critical survey. Opportunity, Challenge and Scope of Natural Products in Medicinal Chemistry - Research Signpost, 661(2), 187–212.

Burda, S., & Oleszek, W. 2001. Antioxidant and antiradical activities of flavonoids. Journal of Agricultural and Food Chemistry, 49(6), 2774–2779. https://doi.org/10.1021/jf001413m

Chang, C. C., Yang, M. H., Wen, H. M., & Chern, J. C. 2002. Estimation of total flavonoid content in propolis by two complementary colometric methods. Journal of Food and Drug Analysis, 10(3), 178–182. https://doi.org/10.38212/2224-6614.2748

Dżugan, M., Sowa, P., Kwasniewska, M., Wesolowska, M., & Czernicka, M. 2016. Physicochemical Parameters and Antioxidant Activity of Bee Honey Enriched With Herbs. Plant Foods for Human Nutrition, 72(1), 74–81. https://doi.org/10.1007/s11130-016-0593-y

Eryuda, F., & Soleha, T. U. 2016. Ekstrak Daun Kluwih (Artocarpus camansi) Dalam Menurunkan Kadar Glukosa Darah Pada Penderita Diabetes Melitus Kluwih Leaf Extract (Artocarpus camansi ) In Lowering Blood Glucose Levels In Patients With Diabetes Melitus. Majority, 5(4), 71–75.

Farsi, E., Shafaei, A., Hor, S. Y., Khadeer Ahamed, M. B., Yam, M. F., Attitalla, I. H., Asmawi, M. Z., & Ismail, Z. 2011. Correlation between enzymes inhibitory effects and antioxidant activities of standardized fractions of methanolic extract obtained from Ficus deltoidea leaves. African Journal of Biotechnology, 10(67), 15184–15194. https://doi.org/10.5897/AJB11.1365

Fathoni, A., Sumarlin, L. O., Putri, J. R., & Fitriana, N. 2020. Antioxidant Activity of Mixed Katuk Leaf Extract and Honey. EduChemia (Jurnal Kimia Dan Pendidikan), 5(2), 168. https://doi.org/10.30870/educhemia.v5i2.7275

Firdausya, H., & Amalia, R. 2020. Aktivitas dan efektivitas antidiabetes pada beberapa tanaman herbal. Farmaka, 18(1), 162–169.

Fitriani, F., Sampepana, E., & Saputra, S. H. 2020. KARAKTERISTIK TANAMAN AKAR BAJAKAH (Spatholobus littoralis Hassk) DARI LOAKULU KABUPATEN KUTAI KARTANEGARA. Jurnal Riset Teknologi Industri, 14(2), 365–376.

Fitrianingsih, S. P., Maulana, I. T., Choesrina, R., Dwiputri, D., & Aprilliani, R. 2016. UJI AKTIVITAS PENGHAMBATAN ALFA AMILASE EKSTRAK DAUN TITHONIA DIVERSIFOLIA SECARA IN VITRO. Prociding SNaPP, 6(1), 108–115.

Foti, M. C. 2007. Antioxidant properties of phenols. Journal of Pharmacy and Pharmacology, 59(12), 1673–1685. https://doi.org/10.1211/jpp.59.12.0010

Ghasemzadeh, A., Jaafar, H. Z. E., & Rahmat, A. 2010. Elevated Carbon Dioxide Increases Contents of Flavonoids and Phenolic Compounds, and Antioxidant Activities in Malaysian Young Ginger (Zingiber officinale Roscoe.) Varieties. Molecules, 15(11), 7907–7922. https://doi.org/10.3390/molecules15117907

Guldas, M., Gurbuz, O., Cakmak, I., Yildiz, E., & Sen, H. 2022. Effects of honey enrichment with Spirulina platensis on phenolics , bioaccessibility , antioxidant capacity and fatty acids. LWT, 153(September 2021), 112461. https://doi.org/10.1016/j.lwt.2021.112461

Halim, B., Syahputra, R. A., Adenin, I., Lubis, H. P., Mendrofa, F., Lie, S., & Nugraha, S. E. 2022. Determination of Phytochemical Constituent, Antioxidant Activity, Total Phenol and Total Flavonoid of Extract Ethanol Phyllanthus emblica Fruit. Pharmacognosy Journal, 14(1), 63–67. https://doi.org/10.5530/pj.2022.14.9

Handajani, A., Betty, R., & Herti, M. 2010. Faktor-Faktor yang Berhubungan Dengan Pola Kematian pada Penyakit Degeneratif di Indonesia. Jurnal Buletin Penelitian Sistem Kesehatan. Buletin Penelitian Sistem Kesehatan, 13(1), 42–45.

Hussein, S. Z., Yusoff, K. M., Makpol, S., & Yusof, Y. A. M. 2011. Antioxidant Capacities and Total Phenolic Contents Increase with Gamma Irradiation in Two Types of Malaysian Honey. Molecules, 16(9), 6378–6395. https://doi.org/10.3390/molecules16086378

Iskandar, D., & Warsidah, W. 2020. Qualitative Phytochemical Screening and Antioxidant Activity of Ethanol Root Extract of Spatholobus littoralis Hassk. The Journal of Food and Medicinal Plants, 1(1), 13–15. https://doi.org/10.25077/jfmp.1.1.13-15.2020

Iskandar, D., Widodo, N., Warsito, Masruri, Rollando, & Antang, Y. P. P. 2022. Phenolic content, antioxidant, cytotoxic of fractions of Spatholobus littoralis Hassk from Kalimantan, Indonesia. Journal of Hunan University Natural Sciences, 49(3), 14–23. https://doi.org/10.55463/issn.1674-2974.49.3.2

Istiqomah, I., & Safitri, D. 2021. Phrmacological Activities of Spatholobus. Jurnal Info Kesehatan, 11(2), 463–469.

Kaushik, P., Singh, G., Khokra, S. L., & Kaushik, D. 2015. Bioassay Guided Fractionation and α-Amylase Inhibitory Activity of Flavanoid Isolated from Pinus roxburghii Sarg. In Natural Products Chemistry & Research (Vol. 03, Issue 04). https://doi.org/10.4172/2329-6836.1000179

Novanty, V., Pangkahila, W., & Dewi, N. N. A. 2021. Administration of ethanol extract of Bajakah tampala ( Spatholobus littoralis Hassk ) stem decreased reactive oxygen species , visceral fat and body weight of obese rats. Neurologico Spinale Medico Chirurgico, 4(1), 32–36. https://doi.org/10.36444/nsmc.v4i1.150

Omidian, K., Rafiei, H., & Bandy, B. 2020. Increased mitochondrial content and function by resveratrol and select flavonoids protects against benzo[a]pyrene-induced bioenergetic dysfunction and ROS generation in a cell model of neoplastic transformation. Free Radical Biology and Medicine, 152(October 2019), 767–775. https://doi.org/10.1016/j.freeradbiomed.2020.01.021

Orak, H. H. 2007. Total antioxidant activities, phenolics, anthocyanins, polyphenoloxidase activities of selected red grape cultivars and their correlations. Scientia Horticulturae, 111(3), 235–241. https://doi.org/10.1016/j.scienta.2006.10.019

Pambudi, D. B., Fajriyah, N. N., & Maharisti, R. A. 2021. Uji Aktivitas Penghambatan ?-Amylase Pada Ekstrak Etanol Daun Adas (Foeniculum vulgare Mill.) Menggunakan Elisa Reader. Urecol Journal. Part C: Health Sciences, 1(1), 1–6. https://doi.org/10.53017/ujhs.1

Pasaribu, S., Wiboworini, B., December, L. K.-I. J. N. S., & 2021, undefined. 2021. Effect of Germinated Black Rice Krisna Extract on Fasting Blood Glucose and Body Weight in Diabetes Mellitus Rats. Ijns.Sums.Ac.Ir, 6(4), 194–200. https://doi.org/10.30476/IJNS.2021.93204.1163.Introduction

Pohorecka, K. 2004. Effect of standardized plant herb extracts on general condition of the honeybee (Apis mellifera L.). Bulletin of the Veterinary Institute in Pulawy, 48(4), 415–419.

Rashidi, W. N. A. S. W. M., Muhammad, N., Abdullah, N., Talip, B. A., & Bahrin, N. 2020. The antioxidant properties and α-amylase inhibition activities of polyphyto mixture with honey formulations. In Food Research (Vol. 4, Issue 6, pp. 2190–2196). https://doi.org/10.26656/fr.2017.4(6).260

Redha, A. 2010. Flavonoid: Struktur, Sifat Antioksidatif dan Peranannya Dalam Sistem Biologis. Jurnal Berlin, 9(2), 196–202. https://doi.org/10.1186/2110-5820-1-7

Saputera, M. M. A., & Ayuchecaria, N. 2018. Uji Efektivitas Ekstrak Etanolik Batang Bajakah Tampala (Spatholobus littoralis Hassk.) Terhadap Waktu Penyembuhan Luka. Jurnal Ilmiah Ibnu Sina, 3(2), 318–327.

Saputera, M. M. A., Marpaung, T. W. A., & Ayuchecaria, N. 2019. KONSENTRASI HAMBAT MINIMUM (KHM) KADAR EKSTRAK ETANOL BATANG BAJAKAH TAMPALA (Spatholobus littoralis Hassk) TERHADAP BAKTERI ESCHERICHIA COLI MELALUI METODE SUMURAN. Jurnal Ilmiah Manuntung, 5(2), 167–173.

Septiana, A. T., Handayani, I., & Winarsi, H. 2019. Aktivitas Antioksidan dan Sifat Fisikokimia Madu Temulawak (Curcuma zanthorrhiza roxb) yang Ditambah Ekstrak Jahe (Zingiber officinale Rosc). Jurnal Aplikasi Teknologi Pangan, 8(4), 155. https://doi.org/10.17728/jatp.4849

Sowa, P., Tarapatskyy, M., Puchalski, C., Jarecki, W., & Dżugan, M. 2019. A novel honey-based product enriched with coumarin from Melilotus flowers. Journal of Food Measurement and Characterization, 13(3), 1748–1754. https://doi.org/10.1007/s11694-019-00092-w

Suharto, I. P. S., Lutfi, E. I., & Rahayu, M. D. 2019. Pengaruh Pemberian Jahe (Zingiber officianale) terhadap Glukosa Darah Pasien Diabetes Mellitus. Jurnal Ilmiah Ilmu Kesehatan, 7(3), 76–83.

Suhendra, C. P., Widarta, I. W. R., & Wiadnyani, A. A. I. S. 2019. PENGARUH KONSENTRASI ETANOL TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK RIMPANG ILALANG (Imperata cylindrica (L) Beauv.) PADA EKSTRAKSI MENGGUNAKAN GELOMBANG ULTRASONIK. Jurnal Ilmu Dan Teknologi Pangan (ITEPA), 8(1), 27. https://doi.org/10.24843/itepa.2019.v08.i01.p04

Sumarlin, L. O., Hadera, M., Chalid, S. Y., & Sukandar, D. 2018. Aktivitas Antioksidan Kombinasi Madu Monoflora dengan Ekstrak Daun Namnam (Cynometra cauliflora L.). Alchemy : Journal of Chemistry, 6(1), 10–17.

Suryanto, R., & Setiawan K, D. 2013. Struktur Data Datawarehouse Tanaman Obat Indonesia Dan Hasil Penelitian Obat Tradisional. Seminar Nasional Sistem Informasi Indonesia, 435–440.

Thalapaneni, N. R., Chidambaram, K. A., Ellappan, T., Sabapathi, M. L., & Mandal, S. C. 2008. Inhibition of carbohydrate digestive enzymes by Talinum portulacifolium (Forssk) leaf extract. Journal of Complementary and Integrative Medicine, 5(1). https://doi.org/10.2202/1553-3840.1120

Wootton-Beard, P. C., Moran, A., & Ryan, L. 2011. Stability of the total antioxidant capacity and total polyphenol content of 23 commercially available vegetable juices before and after in vitro digestion measured by FRAP, DPPH, ABTS and Folin-Ciocalteu methods. Food Research International,44(1)217–224. https://doi.org/10.1016/j.foodres.2010.10.033

Yuhelma, Y., Hasneli, Y., & Nauli, F. A. 2015. Identifikasi dan Analisis Komplikasi Makrovaskuler dan Mikrovaskuler pada Pasien Diabetes Mellitus. Journal Online Mahasiswa, 2(1), 569–579.




DOI: https://doi.org/10.17969/jtipi.v16i1.28522

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