Histologi, Histomorfometri, dan Histokimia Hati Ayam Buras (Gallus gallus domesticus) Selama Periode Sebelum dan Setelah Menetas
Abstract
ABSTRAK. Hati adalah kelenjar terbesar yang terdapat di dalam tubuh dan dapat dianggap sebagai organ pusat dalam pemeliharaan suplai energi. Penelitian ini bertujuan untuk mengetahui histologi, histomorfometri, dan histokimia sebaran glikogen pada hati ayam buras sebelum dan sesudah menetas. Sampel penelitian dibagi menjadi empat kelompok tingkat umur berbeda dan masing-masing kelompok berjumlah enam hati ayam. Hasil penelitian menunjukkan perbedaan nyata (P<0,05) pertumbuhan hati ayam buras secara makroskopis. Pada setiap perkembangan sudah terdapat hepatosit berbentuk poligon yang memiliki inti sel bulat di tengah hepatosit, terdapat juga vena central yang dilapisi sel endotel pipih selapis serta didapati sinusoid yang berada diantara hepatosit, dan juga ditemukan Kupffer’s cell hanya pada hati ayam setelah menetas hari ke-7. Hasil pengukuran diameter vena central hati ayam buras hari ke-7, hari ke-14, hari ke- 20 sebelum menetas dan hari ke-7 setelah menetas berturut-turut adalah 17,70 ± 1,16 µm; 60,32 ± 2,22 µm; 49,316 ± 1,93 µm; dan 129,11 ± 7,43 µm. Hasil histokimia sebaran glikogen pada hati ayam buras hari ke-14, hari ke- 20 sebelum menetas dan hari ke-7 setelah menetas berturut-turut adalah 1,44 ± 0,93; 2,27 ± 0,74; dan 2,77 ± 0,15. Dapat disimpulkan, bahwa diameter vena central dan sebaran glikogen meningkat seiring pertumbuhan ayam buras.
(Histological, histomorphometrical, and histochemical of liver on native chicken (Gallus gallus domesticus) during pre and posthatch period)
ABSTRACT. The liver is the largest gland in the body and considered as a central organ in maintaining energy supplies. The study aimed to determine the histologic, histomorphometric and histochemical of glycogen distribution in the liver of native chicken during pre and posthatching period.. The study sample were divided into four different age groups and each group consisted of six chicken livers. The results showed a significant difference (P<0,05) in native chicken liver growth macroscopically. In every development there are already polygonal hepatocytes that have a round cell nucleus in the middle of the hepatocytes, there is also a central vein that is coated with a layer of flat endothelial cells and also sinusoids that are between the hepatocytes, and kupffer's cells also found in chicken liver 7th day posthatching. The results measurements of the central vein diameter in liver native chickens on the 7th, 14th, 20th pre hatching period and 7th post hatching were 17.70 ± 1.16 µm; 60.32 ± 2.22 µm; 49.32 ± 1.93 µm; and 129.11 ± 7,43 µm; The measure results of the glycogen distribution in liver native chickens on the 14th, 20th pre hatching period and 7th post hatching were 1,44 ± 0,93; 2,27 ± 0,74; and 2,77 ± 0,15. It can be concluded, that central vein diameter and glycogen distribution were increased by growth of native chickens.
Keywords
Full Text:
PDFReferences
Al-Abdulla, M.A.A., 2015. Histological and histochemical study of the liver of Iraqi local ducks. Bas. J. Vet. Res. 14(1): 70-78.
Al-Samawy, E.R.M., Jarad, A.S., Muhamed, A.A., 2016. Histo-morphometric and histochemical comparative study of the liver in collard dove (Fricaldsky), ruddy shelduck (Pallas) in South Iraq. Bas. J. Vet. Res. 15(1): 260-270.
Amiruddin., Siregar, T.N., Hamdan, Azhari, Jalaluddin, Zulkifli, Rahman, A., 2014. Pengaruh pemberian ekstrak hipofisa sapi terhadap peningkatan produktivitas ayam petelur pada fase akhir produksi. Jurnal Kedokteran Hewan. 8(1): 80-84.
Attia, H.F., Soliman, S.M., 2005. Histological and histochemical studies on the ostrich’s liver. Benha Veterinary Medical Journal, 16(2): 1-11.
Bellairs, R., Osmond, M., 2014. The Atlas of Chick Development. 3th ed. Elsevier, Kidlington, Oxford.
Bhadoria, P., Nagar, M., Bahrioke, V., Bhadoria, A.S., 2015. Effect of Ethephon on the liver in albino rats: a histomorphoetric study. Biomed Journal, 38(5): 421-427.
Cho, A., Okazumi, S., Makino, H., Miura, F., Ohira, G., Yoshinaga, Y., Toma, T., Kudo, H., Matsubara, K., Ryu, M., and Ochiai, T., 2004. Relation between hepatic and portal veins in the right paramedian sector: proposal for anatomical reclassification of the liver. World Journal of Surgery. 28(1): 8-12.
Clark, F.D., 2005. Normal bird, a review of avian anatomy. Avian Advice, 7(1): 1-3.
El-Zoghby, I.M.A., 2005. Pre and post hatching development of the quail’s liver. Zagazig Veterinary Journal, 33(1): 185-193.
Fanani, A.F., Suthama, N., Sukamto, B., 2016. Efek penambahan bunga dahlia sebagai sumber inulin terhadap kecernaan protein dan produktivitas ayam lokal persilangan. Jurnal Kedokteran Hewan. 10(1): 58-62.
Faraj, S.S., Al-Bairuty, G.A., 2016. Morphological and histological study of the liver in migratory starling bird (Sturnus vulgaris). Al-Mustansiriyah Journal of Science, 27(5): 11-16.
Foye, O.T., Uni, Z. and Ferket, P.R., 2006. Effect of in ovo feeding egg white protein, hydroxyl- methylbutyrate, and carbohydrates on glycogen status and neonatal growth of turkeys. J. Poult. Sci. 85(1): 1185-1192.
Halawa, E., 2013. Kecernaan ransum mengandung berbagai tingkat bungkil inti sawit ditambahkan hemicell pada itik raja umur 8 minggu. Jurnal Peternakan Integratif. 1(2): 15-23.
Hamodi, H.M., Abed, A.A., Taha, A.M., 2013. Comparative anatomical, histological and histochemical study of the liver in three species of birds. Raf. J. Sci. 24(5): 12-23.
Ibrahim, R.S., Hussein, A.A., Al-kelaby, W.J.A., 2016. Histomorphological study of the liver in local Moorhen birds (Gallinula chloropus). Kufa Journal for Veterinary Medical Sciences. 7(2): 187-192.
Iskandar, S., 2006. Strategi pengembangan ayam lokal. Wartazoa. 16(4): 190-197.
Konig, H.E., Korbel, R. and Liebich, H.G., 2016. Avian Anatomy: Textbook and Colour Atlas. 2nd ed. 5M Publishing, Sheffield, UK.
Kornasio, R., Halevy, O., Kedar, O., Uni, Z., 2011. Effect of in ovo feeding and its interaction with timing of first feed on glycogen reserves, muscle growth and body weight. Poult. Sci. 90(7): 1467-1477.
Kusumawati, A., Febriany, R., Hananti, S., Dewi, M.S., Istiyawati, N., 2016. Perkembangan embrio dan penentuan jenis kelamin DOC (Day-Old Chicken) ayam jawa super. Jurnal Sain Veteriner. 34(1): 29-41.
Mak, K.M., Kwong, A.J., Chu, E., Hoo, N.M., 2012. Hepatic steatosis, fibrosis, and cancer in elderly cadavers. Anat Rec. 295(1): 40-50.
Mclelland, J., 1990. A Colour Atlas of Avian Anatomy. Wolfe Publishing, Aylesbury, England.
Mobasheri, A., Csaki, C., Clutterbuck, A.L., Rahmanzadeh, M., Shakibaei, M., 2009. Mesenchymal stem cells in connective tissue engineering and regenerative medicine: Applications in cartilage repair and osteoarthritis therapy. Histology Histopathology, 24(1): 347-366.
Mobini, B., 2014. Histological study of the gall bladder of the common quail (Coturnix coturnix). Veterinarni Medicina, 59(5): 261-264.
Murwani, R., 2010. Broiler Modern. Widya Karya. Semarang.
Nguyen, H.H., Tumpey, T.M., Park, H.J., Byun, Y.H. Tran, L.D., Nguyen, V.D., Kilgore, P.E., Czerkinsky, C., Katz, J.M., Seong, B.L., Song, J.M., Kim, Y.B., Do, H.T., Nguyen, T., Nguyen, C.V., 2010. Prophylactic and therapeutic efficacy of avian antibodies against influenza virus H5N1 and H1N1 in mice. PloS ONE, 5, e10152.
Petrovova, E., Purzyc, H., Mazensky, D., Luptakova, L., Torma, N., Sopoliga, I., Sedmera, D., 2013. Morphometric alterations, steatosis, fibrosis and active caspase-3 detection in carbamate bendiocarb treated rabbit liver. Environ. Toxicol. 30(2): 212-222.
Pujianti, N.A., Jaelani, A., Widaningsih, N,. 2013. Penambahan tepung kunyit (Curcuma domestica) dalam ransum terhadap daya cerna protein dan bahan kering pada ayam pedaging. Ziraa’ah. 36(1): 49-59.
Rahmi, E., 2008. Distribusi glikogen pada lambung monyet ekor panjang (Macaca fascricularis) selama periode sebelum dan setelah lahir. J. Kedokteran Hewan. 2(2): 176-184.
Scanes, C. G., 2015. Sturkie’s Avian Physiology. 6th ed. Elsevier. Jamestown Road, London.
Selman, H. A., 2013. Morphological and histological study for liver in local coot birds Fulica atra. Bas. J. Vet. Res. 12(1): 152-158.
Si-tayeb, K., Lemaigre, F.P., Duncan, S.A., 2010. Organogenesis and development of the liver. Developmental Cell. 18: 175-189.
Stapleton, D., Nelson, C., Parsawar, K., McClain, D., Gilbert-Wilson, R., Barker, E., Rudd, B., Brown, K., Hendrix, W., O’Donnell, P., Parker, G., 2010. Analysis of hepatic glycogen-associated proteins. Proteomics. 10(1): 2320-2329.
Stornelli, M.R., Ricciardi, M.P., Giannessi, E., Coli, A., 2006. Morphological and histological study of the ostrich (Struthio camelus L.) liver and biliary system . Lit. J. Anat. Embryol. 111(1): 1-7.
Suvarna, S.K., Layton, C., Bancroft, J.D., 2019. Bancroft’s Theory and Practice of Histological Techniques. 8th ed. Elsevier, China.
Wahyuni, S., Agungpriyono, S., Agil, M., Yusuf, T.L., 2012. Histologi dan Histomorfometri Testis dan Epididimis Muncak (Muntiacus muntjak muntjak) pada Periode Ranggah Keras. Jurnal Veteriner. 13(3): 211-219.
Wahyuni, S., Zuchri, Hamny, Jalaluddin, M., Adnyane, I.K.M., 2015. Studi histokimia sebaran karbohidrat usus biawak air (Varanus salvator). Acta Veterinaria Indonesiana, 3(2): 77-84.
Wallach, M. G., Webby, R. J., Islam, F., Walkden-Brown, S., Emmoth, E., Feinstein, R., Gronvik, K. O., 2011. Cross-protection of chicken immunoglobulin Y antibodies against H5N1 and H1N1 viruses passively administered in mice. Clinical Vaccine Immunology, 18(1): 1083-1090.
Wen, J., Zhao, S., He, D., Yang, Y., Li, Y., Zhu, S., 2012. Preparation and characterization of egg yolk immunoglobulin Y specific to influenza B virus. Antiviral Research, 93(1): 154-159.
Yadgary, L., Yair, R., Uni, Z., 2011. The chick embryo yolk sac membrane expresses nutrient transporter and digestive enzyme genes. Poult. Sci. 90: 1302-1312.
Yaman, A., 2011. Ayam Kampung Unggul. Penebar Swadaya, Jakarta.
Yoshida, K., Yasuda, M., Nasu, T., Murakami, T., 2010. Scanning electron microscopic study of vascular and biliary casts in chicken and duck liver. J. Vet. Med. Sci. 72(1): 925-928.
DOI: https://doi.org/10.17969/agripet.v20i2.16011
Article Metrics
Abstract view : 0 timesPDF - 0 times
Refbacks
- There are currently no refbacks.
Copyright (c) 2020 Jurnal Agripet
License URL: http://www.jurnal.unsyiah.ac.id/agripet/about/submissions#copyrightNotice
Copyright© 2000-2024 | ISSN: 1411-4623 | EISSN: 2460-4534
Jurnal Agripet is licensed under a Creative Commons Attribution 4.0 International License.
Published by:
Animal Husbandry Department, The Faculty of Agriculture, Universitas Syiah Kuala
associated with Animal Scientist's Society of Indonesia (HILPI).
Jl. Tgk. Hasan Krueng Kalee No. 3, Kopelma Darussalam,
Banda Aceh, 23111, Indonesia.
Phone: +62-81383736633
Email: jurnalagripet@usk.ac.id
Online Submissions & Guidelines | Editorial Policies | Contact | Statistics | Indexing | Citations