Aktivitas Enzim dan Kecernaan Pakan Domba yang Disuplementasi Complete Rumen Modifier (CRM)

Tri Laras Wigati, Fransiska Maria Suhartati, Sri Rahayu, Muhamad Bata

Abstract


ABSTRACT. Penelitian bertujuan mengkaji pengaruh suplementasi complete rumen modifier (CRM) terhadap aktivitas enzim rumen dan kecernaan pakan domba. Delapan belas ekor domba jantan lokal umur 6 – 8 bulan dengan bobot rata-rata 18,60±2,11 kg dibagi menjadi 6 kelompok berdasarkan bobot badan. Masing-masing kelompok tersebut diacak untuk mendapatkan satu dari tiga perlakuan sehingga penelitian menggunakan rancangan acak kelompok (RAK). Konsumsi bahan kering yang diberikan adalah 4% dari bobot badan domba dengan imbangan bahan kering jerami padi amoniasi dan konsentrat adalah 60 dan 40%. Perlakuan yang diuji adalah penambahan CRM 0, 1, dan 2% dari bahan kering ransum untuk P1, P2, dan P3. Peubah yang diamati meliputi aktivitas protease, dan selulase rumen, kecernaan bahan kering, protein kasar, dan serat kasar. Hasil penelitian menunjukkan bahwa suplementasi CRM menurunkan aktivitas protease rumen secara kuadrater (P<0,01) dengan aktivitas terendah pada taraf CRM 1,16%. Aktivitas selulase meningkat secara kuadrater (P<0,01) dengan aktivitas tertinggi pada taraf CRM 1,06% dengan aktivitas enzim sebesar 0,37 U/mg. Suplementasi CRM tidak berpengaruh (P>0,05) terhadap kecernaan bahan kering pakan. Kecernaan protein kasar dan serat kasar meningkat secara kuadratik (P<0,01) dengan kecernaan tertinggi dicapai pada taraf CRM 1,43% dan 1,69%. Kesimpulan penelitian, suplementasi CRM sebesar 1% - 2% mampu meningkatkan aktivitas enzim rumen dan kecernaan pakan pada domba. Taraf CRM 1% (P2) merupakan taraf terbaik untuk menghasilkan aktivitas enzim rumen dan kecernaan nutrien secara optimal. 

 

(Ruminal enzymes activity and nutrient digestibility of sheep supplemented with Complete Rumen Modifier (CRM)) 

ABSTRAK. This research was carried out to study the supplementation of complete rumen modifier (CRM) to improve feed digestibility and rumen enzymes activity in lambs. Eighteen male local lambs age of 6 – 8 month with average body weight 18.60±2.11 kg were grouped into 6 blocks by initial body weight. Each group was randomly got one out of three treatments, so the experiment used randomized block design. Dry matter intake (DMI) was 4% of body weight which consisted of concentrate and ammoniated rice straw with ratio 60:40%. As a treatment were the addition of CRM to the concentrate of 0, 1, and 2% based on the diet for P1, P2, and P3 respectively. The variables measured were rumen enzymes activity (protease, and cellulose) and nutrient digestibility (dry matter, crude protein, and crude fiber). The result showed that CRM supplementation quadratically (P<0.01) decreased protease activity with the lowest activity at CRM 1,16% but increase cellulose activity quadratically (P<0.01) optimum at level CRM 1,06%. CRM supplementation had no effect (P>0.05) on dry matter digestibility. CRM supplementation quadratically (P<0.01) improved crude protein dan crude fiber digestibility optimum at CRM 1,47% and 1,69% respectively. In conclusion, CRM at a dose 1% - 2% can improve ruminal enzymes and nutrient digestibility in sheep. CRM dose 1% (P2) was the best dose to optimize specific enzymes activity and nutrient digestibility.

Keywords


CRM; digestibility; domba; enzim-rumen; flavonoid; kecernaan; rumen-enzymes; sheep

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References


Abdel-Raheem, S. M., Hassan, E. H. 2021. Effects of dietary inclusion of Moringa oleifera leaf meal on nutrient digestibility, rumen fermentation, ruminal enzyme activities and growth performance of buffalo calves. Saudi J. Biol Sci. 28: 4430. doi:10.1016/j.sjbs.2021.04.037

Abdel Raheem, S., Hassan, E., Farghaly, M. 2018. Effect of dietary concentrate to roughage ratio on nutrient digestibility, rumen fermentation, growth performance and serum acute phase protein in growing buffalo calves. Egyptian J. Nutr. Feeds. 21: 15. doi:10. 21608/ejnf.2018.75381

AOAC. 1990. Official Methods of Analysis. In: AOAC Washington, DC.

Bach, A., Calsamiglia, S., Stern, M. D. 2005. Nitrogen Metabolism in The Rumen. J. Dairy Sci. 88: 9.

Balcells, J., Aris, A., Serrano, A., Seradj, A. R., Crespo, J., Devant, M. 2012. Effects of an extract of plant flavonoids (bioflavex) on rumen fermentation and performance in heifers fed high-concentrate diets. J. Anim.Sci. 90: 4975. doi:10.2527/ jas.2011-4955

Callaway, E. S., Martin, S. A. 1997. Effects of a Saccharomyces cerevisiae Culture on Ruminal Bacteria that Utilize Lactate and Digest Cellulose. J. Dairy Sci. 80: 2035. doi:10.3168/jds.S0022-0302(97) 76148-4

Camassola, M., Dillon, A. 2012. Cellulase determination: modifications to make the filter paper assay easy, fast, practical and efficient. Open Access Scientific Reports. 1:1.

Chen, H., Wang, C., Huasai, S., Chen, A. 2021. Effects of dietary forage to concentrate ratio on nutrient digestibility, ruminal fermentation and rumen bacterial composition in Angus cows. Paper presented at the Scientific Reports.

Chen, L., Shen, Y., Wang, C., Ding, L., Zhao, F., Wang, M., Wang, H. 2019. Megasphaera elsdenii Lactate degradation pattern shifts in rumen acidosis models. Frontiers in Microbiology. 10: 1. doi:10.3389/fmicb. 2019.00162

Cole, H.H., Ronning, M. 1974. Animal Agricultural The Biology of Domestic Animals and Their Use by Man. W.H. Freeman &Co San Fransisco.

Coleman, G. S., Laurie, J. I., Bailey, J. E., Holdgate, S. A. 1976. The cultivation of cellulolytic protozoa isolated from the rumen. J. General Microbiol. 95: 144. doi:10.1099/00221287-95-1-144

Dhia, K. S., Kamil, K. A., Tanuwira, H. 2019. Kecernaan dan Fermentabilitas Substrat Kombinasi Mineral-Fungi dalam Rumen. J. Ilmiah Peternakan Terpadu. 7: 217. doi:10.23960/jipt.v7i2.p217-222

Gijzen, H. J., Lubberding, H. J., Gerhardus, M. J. T., Vogels, G. D. 1988. Contribution of rumen protozoa to fibre degradation and cellulase activity in vitro. FEMS Microbiology Letters. 53, 35. doi:10.1016/ 0378-1097(88)90010-9

Hassan, T. M., Ahmed-Farid, O. A., Abdel-Fattah, F. A. 2020. Effects of Different Sources and Levels of Tannins on Live Performance and Antioxidant Response of Ossimi Lambs. The J. Agric. Sci. 158: 339.

Hassan, F. U., Arshad, M. A., Li, M., Rehman, M. S. U., Loor, J. J., Huang, J. 2020. Potential of mulberry leaf biomass and its flavonoids to improve production and health in ruminants: Mechanistic insights and prospects. Animals. 10: 1. doi:10.3390/ani 10112076

Hernández, J., Benedito, J. L., Abuelo, A., Castillo, C. 2014. Ruminal acidosis in feedlot: From aetiology to prevention. Sci. World J. doi:10.1155/2014/702572

Jadhav, R. V., Chaudhary, L. C., Agarwal, N., Kamra, D. N. 2018. Influence of Moringa oleifera Foliage Supplementation on Feed Intake, Rumen Fermentation and Microbial Profile of Goats. Indian J. Anim. Sci. 88: 458.

Jouany, J. P., Ushida, K. 1999. The Role of Protozoa in Feed Digestion-Review. Asian-Australas J. Anim. Sci. 12: 113. doi.org/ 10.5713/ajas.1999.113

Karto, A. A. 1999. Peran dan kebutuhan sulfur pada ternak ruminansia. Wartazoa. 8: 38.

Khasrad, Rusdimansyah. 2012. Pengaruh Imbangan Konsentrat-Jerami Padi Amoniasi dan Lama Penggemukan terhadap Bobot Badan dan Kualitas Fisik Daging Sapi Pesisir. JITV. 17: 152.

Ku-Vera, J. C., Jiménez-Ocampo, R., Valencia-Salazar, S. S., Montoya-Flores, M. D., Molina-Botero, I. C., Arango, J., Solorio-Sánchez, F. J. 2020. Role of Secondary Plant Metabolites on Enteric Methane Mitigation in Ruminants. Front. Vet. Sci. 7: 1. doi:10.3389/fvets.2020.00584

Kumar, S., Pandey, A. K. 2013. Chemistry and Biological Activities of Flavonoids: An Overview. Sci. World J. 1. doi:10.2307/j.ctt1w0ddx8.35

Lechartier, C., Peyraud, J. L. 2010. The effects of forage proportion and rapidly degradable dry matter from concentrate on ruminal digestion in dairy cows fed corn silage-based diets with fixed neutral detergent fiber and starch contents. J Dairy Sci. 93: 666. doi:10.3168/jds.2009-2349

Lee, S. S., Shin, K. J., Kim, W. Y., Ha, J. K., Han, I. K. 1999. The Rumen Ecosystem: As a Fountain Source of Nobel Enzymes - Review. Asian Journal of Animal Sciences. 12: 988.

Li, M. M., White, R. R., Guan, L. L., Harthan, L., Hanigan, M. D. 2021. Metatranscriptomic analyses reveal ruminal pH regulates fiber degradation and fermentation by shifting the microbial community and gene expression of carbohydrate-active enzymes. Animal Microbiome. 3. doi:10.1186/s425 23-021- 00092-6

Ma, T., Chen, D. D., Tu, Y., Zhang, N. F., Si, B. W., Diao, Q. Y. 2017. Dietary supplementation with mulberry leaf flavonoids inhibits methanogenesis in sheep. Anim. Sci. J. 88: 72. doi:10.1111/ asj.12556

Manh, N. S. 2012. Effect of eucalyptus (Camaldulensis) leaf meal powder on rumen fermentation characteristics in cattle fed on rice straw. Afr. J Agric. Res. 7: 1997-2003. doi:10.5897/ajar 11.1347

Nagaraja, T. G., Titgemeyer, E. C. 2007. Ruminal acidosis in beef cattle: The current microbiological and nutritional outlook. J. Dairy Sci. 90: 17-38. doi:10.3168/jds.2006-478

Neiva, M. C., Schultz, E. B., Sousa, L. M., Oliveira, K. A., Sousa, L. F., Macedo Junior, G. d. L. 2022. Exogenous Enzymes in Sheep Diet: Nutritional and Physiological Parameters. Acta Sci. Anim Sci. 44.

Oskoueian, E., Abdullah, N., Oskoueian, A. 2013. Effects of flavonoids on rumen fermentation activity, methane production, and microbial population. BioMed. Res. Inter. 2013: 1-8. doi:10.1155/2013/349129

Patra, A. K., Saxena, J. 2011. Exploitation of dietary tannins to improve rumen metabolism and ruminant nutrition. J. Sci. Food Agric. 91: 24. doi:10.1002/jsfa.4152

Puastuti, W. 2009. Manipulasi bioproses dalam rumen untuk meningkatkan penggunaan pakan berserat. Wartazoa. 19: 180.

Puspitasari, Partama, N. M., I. B. G., Cakra, I. G. L. O. 2015. Pengaruh Suplementasi vitamin mineral terhadap kecernaan nutrien dan produk fermentasi rumen sapi bali yang diberi ransum berbasis rumput gajah. Majalah Ilmiah Peternakan. 18: 83.

Santra, A., Pathak, N. N. 2001. The effect of dietary concentrate level on rumen enzyme profile and ciliate protozoa population in cattle fed wheat straw diet. J. Anim. Feed Sci. 10: 589. doi:10.22358/jafs/68011/ 2001

Schwab, C. G., Broderick, G. A. 2017. A 100-Year Review: Protein and amino acid nutrition in dairy cows. J. Dairy Sci. 100: 10094. doi:10.3168/jds.2017-13320

Seradj, A. R., Abecia, L., Crespo, J., Villalba, D., Fondevila, M., Balcells, J. 2014. The effect of Bioflavex® and its pure flavonoid components on in vitro fermentation parameters and methane production in rumen fluid from steers given high concentrate diets. Anim. Feed Sci. Technol. 197: 85-91. doi:10.1016/ j. anifeedsci. 2014.08.013

Setyani, W., Setyowati, H. 2018. Phytochemical investigation of noni (Morinda citrifolia L.) leaves extract applicated for sunscreen product. Mal. J. Fund. Appl. Sci. 14: 164-167. doi:10.11113/mjfas.v14n1-2.996

Sheikh, G. G., Ganai, A. M., Ahmad Sheikh, A., Mir, D. M. 2019. Rumen microflora, fermentation pattern and microbial enzyme activity in sheep fed paddy straw based complete feed fortified with probiotics. Biological Rhythm Res. 00: 1-12. doi:10.1080/09291016.2019.1644019

Steel, R. G., Torrie, J. H. 1993. Prinsip dan Prosedur Statistika (Pendekatan Biometrik). Gramedia Pustaka Utama. Jakarta.

Suharyanto, Prima, D. A. N. 2020. Penetapan Kadar Flavonoid Total pada Juice Daun Ubi Jalar Ungu (Ipomoea Batatas L.) yang Berpotensi Sebagai Hepatoprotektor dengan Metode Spektrofotometri UV-Vis. Cendekia J. Pharmacy. 4: 110-119.

Sukmawati, N. M. S., Permana, I. G., Kompiang, S. 2011. Pengaruh Complete Rumen Modifier (CRM) dan Calliandra calothyrus terhadap Produktivitas dan Gas Metan Enterik pada Kambing Perah PE. JITV. 16: 173-183.

Suryapratama, W. 2020. Peningkatan Kecernaan Pakan Sapi Potong yang Berbasis Jerami Padi melalui Suplementasi Amonia, Metionin, Lisin, Kasein, dan Isobutirat secara in vitro. Teknologi dan Agribisnis Peternakan VII–Webinar: Prospek Peternakan di Era Normal Baru Pasca Pandemi COVID-19. Purwokerto: Fakultas Peternakan Universitas Jenderal Soedirman.

Thalib, A., Situmorang, P., Mathius, I. W., Widiawati, Y., Puastuti, W. 2011. The utilization of the complete rumen modifier on dairy cows. JITAA. 36: 137-142. doi:10.14710/jitaa.36.2.137-142

Thalib, A., Widiawati, Y., Haryanto, B. 2010. Penggunaan complete rumen modifier (CRM) pada ternak domba yang diberi hijauan pakan berserat tinggi. JITV. 15: 97-104.

Towaha, J., Balittri. 2013. Kandungan senyawa kimia pada daun teh (Camellia sinensis). Warta Penelitian dan Pengembangan Tanaman Industritian dan Pengembangan Tanaman Industri. 19: 12-16.

Vyas, D., Uwizeye, A., Mohammed, R., Yang, W. Z., Walker, N. D., Beauchemin, K. A. 2014. The effects of active dried and killed dried yeast on subacute ruminal acidosis, ruminal fermentation, and nutrient digestibility in beef heifers. J. Anim. Sci. 92: 724-732. doi:10.2527/ jas.2013-7072

Wallace, R. J. 1996. The proteolytic systems of ruminal microorganisms. Ann Zootech. 45: 301-308.

Wiyatna, M. F., Hernaman, I. 2016. Pengaruh suplementasi metionin-CPO dalam ransum terhadap performa domba. J. Ilmu Ternak. 16: 56-58.

Yanuartono, Y., Nururrozi, A., Indarjulianto, S., Purnamaningsih, H. 2019. Peran Protozoa pada Pencernaan Ruminansia dan Dampak terhadap Lingkungan. J. Ternak Tropika. 20:16-28.

Yoon, I. K., Stern, M. D. 1996. Effects of Saccharomyces cerevisiae and Aspergillus oryzae Cultures on Ruminal Fermentation in Dairy Cows. J. Dairy Sci. 79: 411-417. doi:10.3168/jds.S0022-0302(96) 76380-4

Yulistiani, D., Puastuti, W., Widiawati, Y. 2017. In Vitro Digestibility and Rumen Fermentation of Grass or Rice Straw Basal Diet with or Without Complete Rumen Modifier Supplementation. Proceedings of International Seminar on Livestock Production and Veterinary Technology.

Zhan, J., Liu, M., Su, X., Zhan, K., Zhang, C., Zhao, G. 2017. Effects of alfalfa flavonoids on the production performance, immune system, and ruminal fermentation of dairy cows. Asian-Australas. J. Anim. Sci. 30: 1416-1424. doi:10.5713/ ajas.16.0579.




DOI: https://doi.org/10.17969/agripet.v23i1.26725

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