Pertumbuhan dan Produksi Bakteriosin Lactobacillus fermentum Asal Dangke pada Media Whey Dangke
Abstract
ABSTRAK. Dangke merupakan makanan khas Enrekang, Sulawesi Selatan. Dangke menghasilkan hasil sampingan yang disebut whey. Komponen nutrisi yang terkandung dalam whey dapat digunakan oleh bakteri asam laktat (BAL) untuk pertumbuhannya. Salah satu BAL yang dapat memproduksi bakteriosin yaitu Lactobacillus fermentum (L. fermentum). Strain L. fermentum asal dangke, yang digunakan dalam penelitian yaitu A323L, B323K, dan C113L. Hasil penelitian menunjukkan bahwa fase logaritmik dari L. fermentum strain A323L yaitu pada waktu inkubasi 24-28 jam, sedangkan strain B323K dan C113L pada waktu inkubasi 20-24 jam. Zona hambat dari semua strain L. fermentum termasuk kategori lemah pada media pertumbuhan whey dangke, tetapi strain C113L mempunyai daya hambat terbaik.
(The Growth and Production Bacteriocin of bacteria Lactobacillus fermentum using dangke whey as medium)
ABSTRACT. Dangke is a local dairy product of Enrekang, South Sulawesi. Dangke processing produced a by-product called whey. Nutritional components in whey can be utilized by lactic acid bacteria (LAB) as a nutritional source of growth. One of bacteriocin producing lactic acid bacteria is Lactobacillus fermentum (L. fermentum). L. fermentum strain isolated from dangke, which used in this research were A323L, B323K, and C113L. The results showed that logarithmic phase of L. fermentum strain A323L were occurred at the incubation time of 24-28 hours, whilst strain B323K and C113Lwere at 20-24 hours. Inhibition zone of all strain of L. fermentum was categorized as weak in whey dangke medium, but strain C113L was the best among them.
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Abdelbasset, Djamila, K., 2008.Antimicrobial activity of autochthonous lactic acid bacteria strained from Algerian traditional fermented milk “Raïb”.Afr J Biotech.7(16).
Almeida, K., Tamime, A., Oliveira, M., 2008. Acidification rates of probiotic bacteria in Minas frescal cheese whey. LWT Food Sci Technol. 41(2):311-316.
AOAC (Association Official Analitycal Chemistry).,2005a. Official Method of Analysis.18th Ed. Washington D.C.
Arief, I.I., Budiman, C., Jenie, B.S.J., Andreas, E., dan Yuneni, A., 2015a. Plantaricin IIA-1A5 from Lactobacillus plantarum IIA-1A5 displays bactericidal activity against Staphylococcus aureus. BenefMicrobes6(4):603-613.
Arief, I.I., Jenie, B.S.L., Astawan, M., Fujiyama, K., Witarto, A.B., 2015b. Identification and probiotic characteristics of lactic acid bacteria isolated from Indonesian local beef. Asian J Anim Sci.9:25-36.
Charalampopoulos, D., Pandiella, S., Webb, C., 2003. Evaluation of the effect of malt, wheat and barley extracts on the viability of potentially probiotic lactic acid bacteria under acidic conditions. Int J Food Microbiol. 82(2):133-141.
FAO/WHO (Food and Agriculture Organization/World Health Organization) .2002. Guidelines for The Evaluation of Probiotics in Food. London (UK): Ontario.
Fardiaz, S., 1992a.Mikrobiologi Pangan I. PT. Jakarta (ID): Gramedia Pustaka Utama.
Fardiaz, S., 1992b.Analisis Mikrobiologi Pangan.Jakarta (ID): PT. Raja Garfindo Pesada.
Fatma, Soeparno, S., Nurliyani, N., Hidayat, C., Taufik, M., 2013. Karakteristik Whey Limbah Dangke dan Potensinya Sebagai Produk Minuman dengan Menggunakan Lactobacillus acidophilus Fncc 0051 (Characteristics of Wheyfrom Dangke Waste and Its Potential as Beverage Product by using Lactobacillus acidophilus FNCC 0051). J Agritech. 32(04).
Hosni, K., Hassen, I., Sebeic, H., Casabianca, H.,2013. Secondary metabolites from Chrysanthemum coronarium (Garland) flowerheads:Chemical composition and biological activities. Indus Crops Prod. 44:263– 271.
Kalsum, U., Sjofjan, U., 2012. The growth ofLactobacillus fermentumstrained from quail intestine on rice bran medium. J Trop Life Sci.2(3):58-61.
Karpiński,T., Szkaradkiewicz, A.K., 2013. Characteristic of bacteriocines and their application. Pol J Microbiol. 62(3):223-235.
Lavari, L., Lanniello, R., Paez, R., Zotta, T., Cuatrin, A., Reinheimer, J., Parente, E., Vinderola, G., 2016. Growth of Lactobacillus rhamnosus 64 in wheypermeate and study of the effect of mild stresses on survival to spray drying. LWTFood Sci Technol. 63: 322-330.
Lowry, O.H., Rosebrough, N.J., Farrar, A.L., Randall, R.J., 1951. Protein measurement with the folinphenolreagent.J Biol Chem. 193:265-275.
Mukhlisah, A.N., 2017. Kualitas kimia, fisik dan cemaran mikroba dangke sebagai respon terhadap perbedaan suhu pemanasan dan konsentrasi papain. [tesis]. Bogor (ID): Institut Pertanian Bogor.
Pan, X., Chen, F., Wu, T., Tang, H., Zhao, Z., 2009. The acid, bile tolerance and antimicrobial property of Lactobacillus acidophilus NIT.J Food Control.20:598-602.
Panesar, P.S., Kennedy, J.F., Gandhi, D.N., Bunko, K., 2007. Bioutilisation of wheyfor lactic acid production.F Chem.105:1-14.
Singh, R., Sivasubramani, K., Jayalakshmi, S., 2013. Strainion and production of bacteriocin by marine Lactobacillus fermentum SBS001. Int J Curr Microbiol App Sci. 2(4):67-73.
Syah, S.P., Sumantri, C., Arief, I.I., Taufik, E., 2016. Strainion and identification of indigenous lactic acid bacteria by sequencing the 16S rRNA from dangke, a traditional cheese from Enrekang, South Sulawesi. Pakistan J of Nutrition.
DOI: https://doi.org/10.17969/agripet.v17i2.8104
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